Description
Title: | [KO Validated] FSCN1 Polyclonal Antibody (CAB1904) | |
Size: | 100µL | |
Code: | CAB1904 | |
Host Species: | Rabbit | |
Purification: | Affinity purification | |
Isotype: | IgG |
Background
This gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of this gene is known to be regulated by several microRNAs, and overexpression of this gene may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of this gene is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of this gene is located on the long arm of chromosome 15.
Immunogen Information
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 394-493 of human FSCN1 (NP_003079.1). |
GeneID: | 6624 |
SwissProt: | Q16658 |
Synonyms: | FSCN1; FAN1; HSN; SNL; p55; fascin |
Calculated MW: | 54kDa |
Observed MW: | 50kDa, 55kDa |
Applications
Reactivity: | Human, Mouse, Rat |
Tested Applications: | WB; IHC; IF |
Recommended Dilution: | WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200 |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Application Key: | Western blotting Immunohistochemistry Immunofluorescence |
Positive Samples: | U-251MG, HeLa, NCI-H460, HepG2, SH-SY5Y, Mouse brain, Mouse spinal cord |
Cellular Location: | Cell junction, Cell projection, Cytoplasm, cytoskeleton, cytosol, filopodium, invadopodium, microvillus |
Antibody Images
Western blot analysis of extracts of various cell lines, using FSCN1 antibody (CAB1904) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s. | |
Western blot analysis of extracts from normal (control) and FSCN1 knockout (KO) HeLa cells, using FSCN1 antibody (CAB1904) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s. | |
Immunohistochemistry of paraffin-embedded rat stomach using FSCN1 antibody (CAB1904) at dilution of 1:100 (40x lens). | |
Immunofluorescence analysis of rat skin using FSCN1 antibody (CAB1904) at dilution of 1:100. Blue: DAPI for nuclear staining. | |
Immunofluorescence analysis of human skin cells using FSCN1 antibody (CAB1904) at dilution of 1:100. Blue: DAPI for nuclear staining. |
Additional Information
Antibody Type: |
Polyclonal |
Application: |
WB |
Application: |
IHC |
Application: |
IF |
Reactivity: |
Human |
Reactivity: |
Mouse |
Reactivity: |
Rat |
Host Species: |
Rabbit |
Isotype: |
IgG |