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[KO Validated] FSCN1 Polyclonal Antibody (CAB1904)

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CAB1904
  • Western blot - [KO Validated] FSCN1 Polyclonal Antibody (CAB1904)
  • Western blot - [KO Validated] FSCN1 Polyclonal Antibody (CAB1904)
  • Immunohistochemistry - [KO Validated] FSCN1 Polyclonal Antibody (CAB1904)
  • Immunofluorescence - [KO Validated] FSCN1 Polyclonal Antibody (CAB1904)
  • Immunofluorescence - [KO Validated] FSCN1 Polyclonal Antibody (CAB1904)
$553.89

Description

Overview

Title:[KO Validated] FSCN1 Polyclonal Antibody (CAB1904)
Size:100µL
Code:CAB1904
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of this gene is known to be regulated by several microRNAs, and overexpression of this gene may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of this gene is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of this gene is located on the long arm of chromosome 15.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 394-493 of human FSCN1 (NP_003079.1).
GeneID:6624
SwissProt:Q16658
Synonyms:FSCN1; FAN1; HSN; SNL; p55; fascin
Calculated MW:54kDa
Observed MW:50kDa, 55kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:U-251MG, HeLa, NCI-H460, HepG2, SH-SY5Y, Mouse brain, Mouse spinal cord
Cellular Location:Cell junction, Cell projection, Cytoplasm, cytoskeleton, cytosol, filopodium, invadopodium, microvillus

Antibody Images

Western blot - [KO Validated] FSCN1 Polyclonal Antibody (CAB1904)

Western blot analysis of extracts of various cell lines, using FSCN1 antibody (CAB1904) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Western blot - [KO Validated] FSCN1 Polyclonal Antibody (CAB1904)

Western blot analysis of extracts from normal (control) and FSCN1 knockout (KO) HeLa cells, using FSCN1 antibody (CAB1904) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

Immunohistochemistry - [KO Validated] FSCN1 Polyclonal Antibody (CAB1904)

Immunohistochemistry of paraffin-embedded rat stomach using FSCN1 antibody (CAB1904) at dilution of 1:100 (40x lens).

Immunofluorescence - [KO Validated] FSCN1 Polyclonal Antibody (CAB1904)

Immunofluorescence analysis of rat skin using FSCN1 antibody (CAB1904) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence - [KO Validated] FSCN1 Polyclonal Antibody (CAB1904)

Immunofluorescence analysis of human skin cells using FSCN1 antibody (CAB1904) at dilution of 1:100. Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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