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[KO Validated] FASN Polyclonal Antibody (CAB0462)

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CAB0462
  • Western blot - [KO Validated] FASN Polyclonal Antibody (CAB0462)
  • Western blot - [KO Validated] FASN Polyclonal Antibody (CAB0462)
  • Immunohistochemistry - [KO Validated] FASN Polyclonal Antibody (CAB0462)
  • Immunohistochemistry - [KO Validated] FASN Polyclonal Antibody (CAB0462)
  • Immunohistochemistry - [KO Validated] FASN Polyclonal Antibody (CAB0462)
  • Immunohistochemistry - [KO Validated] FASN Polyclonal Antibody (CAB0462)
  • Immunofluorescence - [KO Validated] FASN Polyclonal Antibody (CAB0462)
  • Immunoprecipitation - [KO Validated] FASN Polyclonal Antibody (CAB0462)
$553.89

Description

Overview

Title:[KO Validated] FASN Polyclonal Antibody (CAB0462)
Size:100µL
Code:CAB0462
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 2212-2511 of human FASN (NP_004095.4).
GeneID:2194
SwissProt:P49327
Synonyms:FASN; FAS; OA-519; SDR27X1; fatty acid synthase
Calculated MW:273kDa
Observed MW:275kDa

Applications

Reactivity:Human
Tested Applications:WB; IHC; IF; IP
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:100; IF 1:50 - 1:200; IP 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive Samples:H460
Cellular Location:Cytoplasm, Melanosome

Antibody Images

Western blot - [KO Validated] FASN Polyclonal Antibody (CAB0462)

Western blot analysis of extracts of H460 cells, using FASN antibody (CAB0462) at 1:200 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Western blot - [KO Validated] FASN Polyclonal Antibody (CAB0462)

Western blot analysis of extracts from normal (control) and FASN knockout (KO) 293T cells, using FASN antibody (CAB0462) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Immunohistochemistry - [KO Validated] FASN Polyclonal Antibody (CAB0462)

Immunohistochemistry of paraffin-embedded human colon using FASN antibody (CAB0462) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] FASN Polyclonal Antibody (CAB0462)

Immunohistochemistry of paraffin-embedded human breast cancer using FASN antibody (CAB0462) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] FASN Polyclonal Antibody (CAB0462)

Immunohistochemistry of paraffin-embedded human stomach using FASN antibody (CAB0462) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] FASN Polyclonal Antibody (CAB0462)

Immunohistochemistry of paraffin-embedded human gastric cancer using FASN antibody (CAB0462) at dilution of 1:100 (40x lens).

Immunofluorescence - [KO Validated] FASN Polyclonal Antibody (CAB0462)

Immunofluorescence analysis of A549 cells using FASN antibody (CAB0462). Blue: DAPI for nuclear staining.

Immunoprecipitation - [KO Validated] FASN Polyclonal Antibody (CAB0462)

Immunoprecipitation analysis of 200ug extracts of H460 cells, using 3 ug FASN antibody (CAB0462). Western blot was performed from the immunoprecipitate using FASN antibody (CAB0462) at a dilition of 1:1000.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Application:
IP
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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