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[KO Validated] ErbB2 Polyclonal Antibody (CAB2071)

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SKU:
CAB2071
  • Western blot - [KO Validated] ErbB2 Polyclonal Antibody (CAB2071)
  • Western blot - [KO Validated] ErbB2 Polyclonal Antibody (CAB2071)
  • Immunohistochemistry - [KO Validated] ErbB2 Polyclonal Antibody (CAB2071)
  • Immunohistochemistry - [KO Validated] ErbB2 Polyclonal Antibody (CAB2071)
  • Immunohistochemistry - [KO Validated] ErbB2 Polyclonal Antibody (CAB2071)
  • Immunohistochemistry - [KO Validated] ErbB2 Polyclonal Antibody (CAB2071)
  • Immunohistochemistry - [KO Validated] ErbB2 Polyclonal Antibody (CAB2071)
$553.89

Description

Overview

Title:[KO Validated] ErbB2 Polyclonal Antibody (CAB2071)
Size:100µL
Code:CAB2071
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.

Immunogen Information

Immunogen:A synthetic peptide of human ErbB2
GeneID:2064
SwissProt:P04626
Synonyms:ERBB2; CD340; HER-2; HER-2/neu; HER2; MLN 19; NEU; NGL; TKR1; MLN19; erb-b2 receptor tyrosine kinase 2
Calculated MW:62kDa/70kDa/97kDa/134kDa/136kDa/137kDa
Observed MW:185kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:293T, HepG2, HeLa, Mouse uterus
Cellular Location:Cell membrane, Cytoplasm, Nucleus, Nucleus, Single-pass type I membrane protein, perinuclear region

Antibody Images

Western blot - [KO Validated] ErbB2 Polyclonal Antibody (CAB2071)

Western blot analysis of extracts of various cell lines, using ErbB2 antibody (CAB2071) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Western blot - [KO Validated] ErbB2 Polyclonal Antibody (CAB2071)

Western blot analysis of extracts from normal (control) and ErbB2 knockout (KO) HeLa cells, using ErbB2 antibody (CAB2071) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5min.

Immunohistochemistry - [KO Validated] ErbB2 Polyclonal Antibody (CAB2071)

Immunohistochemistry of paraffin-embedded rat lung using ErbB2 antibody (CAB2071) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] ErbB2 Polyclonal Antibody (CAB2071)

Immunohistochemistry of paraffin-embedded rat ovary using ErbB2 antibody (CAB2071) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] ErbB2 Polyclonal Antibody (CAB2071)

Immunohistochemistry of paraffin-embedded human tonsil using ErbB2 antibody (CAB2071) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] ErbB2 Polyclonal Antibody (CAB2071)

Immunohistochemistry of paraffin-embedded human lung cancer using ErbB2 antibody (CAB2071) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] ErbB2 Polyclonal Antibody (CAB2071)

Immunohistochemistry of paraffin-embedded mouse kidney using ErbB2 antibody (CAB2071) at dilution of 1:100 (40x lens).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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