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[KO Validated] EIF4EBP1 Polyclonal Antibody (CAB1248)

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CAB1248
  • Western blot - [KO Validated] EIF4EBP1 Polyclonal Antibody (CAB1248)
  • Western blot - [KO Validated] EIF4EBP1 Polyclonal Antibody (CAB1248)
  • Immunofluorescence - [KO Validated] EIF4EBP1 Polyclonal Antibody (CAB1248)
$553.89

Description

Overview

Title:[KO Validated] EIF4EBP1 Polyclonal Antibody (CAB1248)
Size:100µL
Code:CAB1248
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Immunogen Information

Immunogen:A synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human EIF4EBP1 (NP_004086.1).
GeneID:1978
SwissProt:Q13541
Synonyms:EIF4EBP1; 4E-BP1; 4EBP1; BP-1; PHAS-I; eukaryotic translation initiation factor 4E-binding protein 1
Calculated MW:12kDa
Observed MW:20kDa, 22kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IF
Recommended Dilution:WB 1:500 - 1:1000; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    
Positive Samples:HL-60, SKOV3, U-87MG, HeLa, Mouse liver, Mouse heart
Cellular Location:N/A

Antibody Images

Western blot - [KO Validated] EIF4EBP1 Polyclonal Antibody (CAB1248)

Western blot analysis of extracts of various cell lines, using EIF4EBP1 antibody (CAB1248) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.

Western blot - [KO Validated] EIF4EBP1 Polyclonal Antibody (CAB1248)

Western blot analysis of extracts from normal (control) and EIF4EBP1 knockout (KO) HeLa cells, using EIF4EBP1 antibody (CAB1248) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Immunofluorescence - [KO Validated] EIF4EBP1 Polyclonal Antibody (CAB1248)

Immunofluorescence analysis of NIH/3T3 cells using EIF4EBP1 antibody (CAB1248) at dilution of 1:100. Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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