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[KO Validated] Eif2ak2 Polyclonal Antibody (CAB5578)

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CAB5578
  • Western blot - [KO Validated] Eif2ak2 Polyclonal Antibody (CAB5578)
  • Western blot - [KO Validated] Eif2ak2 Polyclonal Antibody (CAB5578)
  • Immunohistochemistry - [KO Validated] Eif2ak2 Polyclonal Antibody (CAB5578)
  • Immunofluorescence - [KO Validated] Eif2ak2 Polyclonal Antibody (CAB5578)
$553.89

Description

Overview

Title:[KO Validated] Eif2ak2 Polyclonal Antibody (CAB5578)
Size:100µL
Code:CAB5578
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human Eif2ak2 (NP_002750.1).
GeneID:5610
SwissProt:P19525
Synonyms:EIF2AK2; EIF2AK1; PKR; PPP1R83; PRKR; eukaryotic translation initiation factor 2 alpha kinase 2
Calculated MW:57kDa/62kDa
Observed MW:73kDa, 80kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:100 - 1:200; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:A-549, HeLa, MCF-7, Jurkat, HepG2
Cellular Location:Cytoplasm, Nucleus, perinuclear region

Antibody Images

Western blot - [KO Validated] Eif2ak2 Polyclonal Antibody (CAB5578)

Western blot analysis of extracts of various cell lines, using Eif2ak2 antibody (CAB5578) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Western blot - [KO Validated] Eif2ak2 Polyclonal Antibody (CAB5578)

Western blot analysis of extracts from normal (control) and EIF2AK2 knockout (KO) HeLa cells, using EIF2AK2 antibody (CAB5578) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Immunohistochemistry - [KO Validated] Eif2ak2 Polyclonal Antibody (CAB5578)

Immunohistochemistry of paraffin-embedded human liver using Eif2ak2 antibody (CAB5578) at dilution of 1:100 (40x lens).

Immunofluorescence - [KO Validated] Eif2ak2 Polyclonal Antibody (CAB5578)

Immunofluorescence analysis of U2OS cells using EIF2AK2 antibody (CAB5578) at dilution of 1:100. Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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