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[KO Validated] EEF2K Polyclonal Antibody (CAB5404)

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CAB5404
  • Western blot - [KO Validated] EEF2K Polyclonal Antibody (CAB5404)
  • Western blot - [KO Validated] EEF2K Polyclonal Antibody (CAB5404)
  • Immunofluorescence - [KO Validated] EEF2K Polyclonal Antibody (CAB5404)
$553.89

Description

Overview

Title:[KO Validated] EEF2K Polyclonal Antibody (CAB5404)
Size:100µL
Code:CAB5404
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 406-725 of human EEF2K (NP_037434.1).
GeneID:29904
SwissProt:O00418
Synonyms:EEF2K; HSU93850; eEF-2K; eukaryotic elongation factor 2 kinase
Calculated MW:82kDa
Observed MW:110kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB; IF
Recommended Dilution:WB 1:500 - 1:2000; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    
Positive Samples:HepG2, HeLa, LOVO, 22Rv1, 293T, Mouse spleen
Cellular Location:N/A

Antibody Images

Western blot - [KO Validated] EEF2K Polyclonal Antibody (CAB5404)

Western blot analysis of extracts of various cell lines, using EEF2K antibody (CAB5404) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 40s.

Western blot - [KO Validated] EEF2K Polyclonal Antibody (CAB5404)

Western blot analysis of extracts from normal (control) and EEF2K knockout (KO) HeLa cells, using EEF2K antibody (CAB5404) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Immunofluorescence - [KO Validated] EEF2K Polyclonal Antibody (CAB5404)

Immunofluorescence analysis of A-549 cells using EEF2K antibody (CAB5404). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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