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[KO Validated] EEF1B2 Polyclonal Antibody (CAB6580)

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CAB6580
  • Western blot - [KO Validated] EEF1B2 Polyclonal Antibody (CAB6580)
  • Western blot - [KO Validated] EEF1B2 Polyclonal Antibody (CAB6580)
  • Immunohistochemistry - [KO Validated] EEF1B2 Polyclonal Antibody (CAB6580)
  • Immunohistochemistry - [KO Validated] EEF1B2 Polyclonal Antibody (CAB6580)
  • Immunohistochemistry - [KO Validated] EEF1B2 Polyclonal Antibody (CAB6580)
  • Immunohistochemistry - [KO Validated] EEF1B2 Polyclonal Antibody (CAB6580)
  • Immunofluorescence - [KO Validated] EEF1B2 Polyclonal Antibody (CAB6580)
$553.89

Description

Overview

Title:[KO Validated] EEF1B2 Polyclonal Antibody (CAB6580)
Size:100µL
Code:CAB6580
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a translation elongation factor. The protein is a guanine nucleotide exchange factor involved in the transfer of aminoacylated tRNAs to the ribosome. Alternative splicing results in three transcript variants which differ only in the 5' UTR.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-225 of human EEF1B2 (NP_001032752.1).
GeneID:1933
SwissProt:P24534
Synonyms:EEF1B2; EEF1B; EEF1B1; EF1B; elongation factor 1-beta
Calculated MW:24kDa
Observed MW:33kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:MCF-7, HL-60, HeLa, 293T, Mouse pancreas
Cellular Location:N/A

Antibody Images

Western blot - [KO Validated] EEF1B2 Polyclonal Antibody (CAB6580)

Western blot analysis of extracts of various cell lines, using EEF1B2 antibody (CAB6580) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Western blot - [KO Validated] EEF1B2 Polyclonal Antibody (CAB6580)

Western blot analysis of extracts from normal (control) and EEF1B2 knockout (KO) 293T cells, using EEF1B2 antibody (CAB6580) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Immunohistochemistry - [KO Validated] EEF1B2 Polyclonal Antibody (CAB6580)

Immunohistochemistry of paraffin-embedded mouse brain using EEF1B2 Antibody (CAB6580) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] EEF1B2 Polyclonal Antibody (CAB6580)

Immunohistochemistry of paraffin-embedded rat spinal cord using EEF1B2 Antibody (CAB6580) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] EEF1B2 Polyclonal Antibody (CAB6580)

Immunohistochemistry of paraffin-embedded rat brain using EEF1B2 Antibody (CAB6580) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] EEF1B2 Polyclonal Antibody (CAB6580)

Immunohistochemistry of paraffin-embedded mouse brain using EEF1B2 Antibody (CAB6580) at dilution of 1:100 (40x lens).

Immunofluorescence - [KO Validated] EEF1B2 Polyclonal Antibody (CAB6580)

Immunofluorescence analysis of A549 cells using EEF1B2 antibody (CAB6580). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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