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[KO Validated] DNAJB1 Polyclonal Antibody (CAB5504)

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CAB5504
  • Western blot - [KO Validated] DNAJB1 Polyclonal Antibody (CAB5504)
  • Western blot - [KO Validated] DNAJB1 Polyclonal Antibody (CAB5504)
  • Western blot - [KO Validated] DNAJB1 Polyclonal Antibody (CAB5504)
  • Immunofluorescence - [KO Validated] DNAJB1 Polyclonal Antibody (CAB5504)
  • Immunofluorescence - [KO Validated] DNAJB1 Polyclonal Antibody (CAB5504)
  • Immunofluorescence - [KO Validated] DNAJB1 Polyclonal Antibody (CAB5504)
$553.89

Description

Overview

Title:[KO Validated] DNAJB1 Polyclonal Antibody (CAB5504)
Size:100µL
Code:CAB5504
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-340 of human DNAJB1 (NP_006136.1).
GeneID:3337
SwissProt:P25685
Synonyms:DNAJB1; HSPF1; Hdj1; Hsp40; RSPH16B; Sis1; dnaJ homolog subfamily B member 1
Calculated MW:27kDa/38kDa
Observed MW:39kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IF
Recommended Dilution:WB 1:500 - 1:2000; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    
Positive Samples:293T, MCF-7, BxPC-3, BT-474, SW620, K-562, Mouse thymus, Mouse spleen, Mouse thymus
Cellular Location:Cytoplasm, Nucleus, nucleolus

Antibody Images

Western blot - [KO Validated] DNAJB1 Polyclonal Antibody (CAB5504)

Western blot analysis of extracts of various cell lines, using DNAJB1 antibody (CAB5504) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.

Western blot - [KO Validated] DNAJB1 Polyclonal Antibody (CAB5504)

Western blot analysis of extracts of various cells, using DNAJB1 antibody (CAB5504) at 1:1000 dilution ratio through one-step method.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

Western blot - [KO Validated] DNAJB1 Polyclonal Antibody (CAB5504)

Western blot analysis of extracts from normal (control) and DNAJB1 knockout (KO) 293T cells, using DNAJB1 antibody (CAB5504) at 1:1000 dilution.

Immunofluorescence - [KO Validated] DNAJB1 Polyclonal Antibody (CAB5504)

Immunofluorescence analysis of U2OS cells using DNAJB1 antibody (CAB5504). Blue: DAPI for nuclear staining.

Immunofluorescence - [KO Validated] DNAJB1 Polyclonal Antibody (CAB5504)

Immunofluorescence analysis of C6 cells using DNAJB1 antibody (CAB5504) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence - [KO Validated] DNAJB1 Polyclonal Antibody (CAB5504)

Immunofluorescence analysis of HeLa cells using DNAJB1 antibody (CAB5504) at dilution of 1:100. Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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