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[KO Validated] DFFA Polyclonal Antibody (CAB1032)

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SKU:
CAB1032
  • Western blot - [KO Validated] DFFA Polyclonal Antibody (CAB1032)
  • Western blot - [KO Validated] DFFA Polyclonal Antibody (CAB1032)
  • Immunofluorescence - [KO Validated] DFFA Polyclonal Antibody (CAB1032)
$553.89

Description

Overview

Title:[KO Validated] DFFA Polyclonal Antibody (CAB1032)
Size:100µL
Code:CAB1032
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-331 of human DFFA (NP_004392.1).
GeneID:1676
SwissProt:O00273
Synonyms:DFFA; DFF-45; DFF1; ICAD; DNA fragmentation factor subunit alpha
Calculated MW:29kDa/36kDa
Observed MW:35kDa, 45kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IF
Recommended Dilution:WB 1:500 - 1:2000; IF 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    
Positive Samples:Jurkat, H460, HepG2, Mouse brain, Rat brain
Cellular Location:Cytoplasm

Antibody Images

Western blot - [KO Validated] DFFA Polyclonal Antibody (CAB1032)

Western blot analysis of extracts of various cell lines, using DFFA antibody (CAB1032) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.

Western blot - [KO Validated] DFFA Polyclonal Antibody (CAB1032)

Western blot analysis of extracts from normal (control) and DFFA knockout (KO) HeLa cells, using DFFA antibody (CAB1032) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

Immunofluorescence - [KO Validated] DFFA Polyclonal Antibody (CAB1032)

Immunofluorescence analysis of HeLa cells using DFFA antibody (CAB1032). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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