[KO Validated] DDIT4 Polyclonal Antibody (CAB8086)
- SKU:
- CAB8086
![Western blot - [KO Validated] DDIT4 Polyclonal Antibody (CAB8086)](https://cdn11.bigcommerce.com/s-lr6p52av1n/images/stencil/1280x1280/products/100138/248192/A8086_1__23348.1547242587.jpg?c=2?imbypass=on "Western blot - [KO Validated] DDIT4 Polyclonal Antibody (CAB8086)")
![Western blot - [KO Validated] DDIT4 Polyclonal Antibody (CAB8086)](https://cdn11.bigcommerce.com/s-lr6p52av1n/images/stencil/1280x1280/products/100138/248193/A8086_2__45358.1547242587.jpg?c=2?imbypass=on "Western blot - [KO Validated] DDIT4 Polyclonal Antibody (CAB8086)")
![Western blot - [KO Validated] DDIT4 Polyclonal Antibody (CAB8086)](https://cdn11.bigcommerce.com/s-lr6p52av1n/stencil/11997510-6c9e-0136-509a-71bd3cb7e197/e/607eadb0-ac84-0137-c78f-0242ac11002a/img/loading.svg "Western blot - [KO Validated] DDIT4 Polyclonal Antibody (CAB8086)")
Description
| Title: | [KO Validated] DDIT4 Polyclonal Antibody (CAB8086) | |
| Size: | 100µL | |
| Code: | CAB8086 | |
| Host Species: | Rabbit | |
| Purification: | Affinity purification | |
| Isotype: | IgG |
Background
N/A
Immunogen Information
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-232 of human DDIT4 (NP_061931.1). |
| GeneID: | 54541 |
| SwissProt: | Q9NX09 |
| Synonyms: | DDIT4; Dig2; REDD-1; REDD1; DNA damage inducible transcript 4 |
| Calculated MW: | 25kDa |
| Observed MW: | 35kDa |
Applications
| Reactivity: | Human |
| Tested Applications: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Application Key: | Western blotting |
| Positive Samples: | A-549, K-562, DU145, MCF-7 |
| Cellular Location: | Cytoplasm, Mitochondrion, cytosol |
Antibody Images
| Western blot analysis of extracts of various cell lines, using DDIT4 antibody (CAB8086) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s. |
| Western blot analysis of extracts from normal (control) and DDIT4 knockout (KO) HeLa cells, using DDIT4 antibody (CAB8086) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s. |
![Western blot - [KO Validated] DDIT4 Polyclonal Antibody (CAB8086)](https://cdn11.bigcommerce.com/s-lr6p52av1n/product_images/g/660/A8086_1__23348.jpg)
![Western blot - [KO Validated] DDIT4 Polyclonal Antibody (CAB8086)](https://cdn11.bigcommerce.com/s-lr6p52av1n/product_images/y/591/A8086_2__45358.jpg)
Datasheets
Additional Information
Antibody Type: |
Polyclonal |
Application: |
WB |
Reactivity: |
Human |
Host Species: |
Rabbit |
Isotype: |
IgG |
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