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[KO Validated] DDIT4 Polyclonal Antibody (CAB8086)

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CAB8086
  • Western blot - [KO Validated] DDIT4 Polyclonal Antibody (CAB8086)
  • Western blot - [KO Validated] DDIT4 Polyclonal Antibody (CAB8086)
$553.89

Description

Overview

Title:[KO Validated] DDIT4 Polyclonal Antibody (CAB8086)
Size:100µL
Code:CAB8086
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

N/A

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-232 of human DDIT4 (NP_061931.1).
GeneID:54541
SwissProt:Q9NX09
Synonyms:DDIT4; Dig2; REDD-1; REDD1; DNA damage inducible transcript 4
Calculated MW:25kDa
Observed MW:35kDa

Applications

Reactivity:Human
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:A-549, K-562, DU145, MCF-7
Cellular Location:Cytoplasm, Mitochondrion, cytosol

Antibody Images

Western blot - [KO Validated] DDIT4 Polyclonal Antibody (CAB8086)

Western blot analysis of extracts of various cell lines, using DDIT4 antibody (CAB8086) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Western blot - [KO Validated] DDIT4 Polyclonal Antibody (CAB8086)

Western blot analysis of extracts from normal (control) and DDIT4 knockout (KO) HeLa cells, using DDIT4 antibody (CAB8086) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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