Description
Title: | [KO Validated] DDIT4 Polyclonal Antibody (CAB8086) | |
Size: | 100µL | |
Code: | CAB8086 | |
Host Species: | Rabbit | |
Purification: | Affinity purification | |
Isotype: | IgG |
Background
N/A
Immunogen Information
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-232 of human DDIT4 (NP_061931.1). |
GeneID: | 54541 |
SwissProt: | Q9NX09 |
Synonyms: | DDIT4; Dig2; REDD-1; REDD1; DNA damage inducible transcript 4 |
Calculated MW: | 25kDa |
Observed MW: | 35kDa |
Applications
Reactivity: | Human |
Tested Applications: | WB |
Recommended Dilution: | WB 1:500 - 1:2000 |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Application Key: | Western blotting |
Positive Samples: | A-549, K-562, DU145, MCF-7 |
Cellular Location: | Cytoplasm, Mitochondrion, cytosol |
Antibody Images
Western blot analysis of extracts of various cell lines, using DDIT4 antibody (CAB8086) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s. | |
Western blot analysis of extracts from normal (control) and DDIT4 knockout (KO) HeLa cells, using DDIT4 antibody (CAB8086) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s. |
Additional Information
Antibody Type: |
Polyclonal |
Application: |
WB |
Reactivity: |
Human |
Host Species: |
Rabbit |
Isotype: |
IgG |