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[KO Validated] CSPG4 Polyclonal Antibody (CAB3592)

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CAB3592
  • Western blot - [KO Validated] CSPG4 Polyclonal Antibody (CAB3592)
  • Western blot - [KO Validated] CSPG4 Polyclonal Antibody (CAB3592)
$553.89

Description

Overview

Title:[KO Validated] CSPG4 Polyclonal Antibody (CAB3592)
Size:100µL
Code:CAB3592
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1950-2210 of human CSPG4 (NP_001888.2).
GeneID:1464
SwissProt:Q6UVK1
Synonyms:CSPG4; HMW-MAA; MCSP; MCSPG; MEL-CSPG; MSK16; NG2; chondroitin sulfate proteoglycan 4
Calculated MW:250kDa
Observed MW:320kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IF
Recommended Dilution:WB 1:500 - 1:2000; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    
Positive Samples:A-375, HeLa
Cellular Location:Apical cell membrane, Cell projection, Extracellular side, Single-pass type I membrane protein, lamellipodium membrane

Antibody Images

Western blot - [KO Validated] CSPG4 Polyclonal Antibody (CAB3592)

Western blot analysis of extracts of various cell lines, using CSPG4 antibody (CAB3592) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Western blot - [KO Validated] CSPG4 Polyclonal Antibody (CAB3592)

Western blot analysis of extracts from normal (control) and CSPG4 knockout (KO) HeLa cells, using CSPG4 antibody (CAB3592) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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