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[KO Validated] CSNK1E Polyclonal Antibody (CAB1796)

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CAB1796
  • Western blot - [KO Validated] CSNK1E Polyclonal Antibody (CAB1796)
  • Western blot - [KO Validated] CSNK1E Polyclonal Antibody (CAB1796)
  • Immunohistochemistry - [KO Validated] CSNK1E Polyclonal Antibody (CAB1796)
  • Immunohistochemistry - [KO Validated] CSNK1E Polyclonal Antibody (CAB1796)
  • Immunohistochemistry - [KO Validated] CSNK1E Polyclonal Antibody (CAB1796)
  • Immunohistochemistry - [KO Validated] CSNK1E Polyclonal Antibody (CAB1796)
  • Immunofluorescence - [KO Validated] CSNK1E Polyclonal Antibody (CAB1796)
$553.89

Description

Overview

Title:[KO Validated] CSNK1E Polyclonal Antibody (CAB1796)
Size:100µL
Code:CAB1796
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is a serine/threonine protein kinase and a member of the casein kinase I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. This protein has been shown to phosphorylate period, a circadian rhythm protein. Two transcript variants encoding the same protein have been found for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 247-416 of human CSNK1E (NP_689407.1).
GeneID:1454
SwissProt:P49674
Synonyms:CSNK1E; CKIepsilon; HCKIE; casein kinase 1 epsilon
Calculated MW:47kDa
Observed MW:47kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF; IP
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200; IP 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive Samples:HeLa, 293T
Cellular Location:Cytoplasm, Nucleus

Antibody Images

Western blot - [KO Validated] CSNK1E Polyclonal Antibody (CAB1796)

Western blot analysis of extracts of various cell lines, using CSNK1E antibody (CAB1796) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Western blot - [KO Validated] CSNK1E Polyclonal Antibody (CAB1796)

Western blot analysis of extracts from normal (control) and CSNK1E knockout (KO) HeLa cells, using CSNK1E antibody (CAB1796) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

Immunohistochemistry - [KO Validated] CSNK1E Polyclonal Antibody (CAB1796)

Immunohistochemistry of paraffin-embedded rat pancreas using CSNK1E Antibody (CAB1796) at dilution of 1:200 (40x lens).

Immunohistochemistry - [KO Validated] CSNK1E Polyclonal Antibody (CAB1796)

Immunohistochemistry of paraffin-embedded mouse brain using CSNK1E Antibody (CAB1796) at dilution of 1:200 (40x lens).

Immunohistochemistry - [KO Validated] CSNK1E Polyclonal Antibody (CAB1796)

Immunohistochemistry of paraffin-embedded rat pancreas using CSNK1E Antibody (CAB1796) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] CSNK1E Polyclonal Antibody (CAB1796)

Immunohistochemistry of paraffin-embedded mouse brain using CSNK1E Antibody (CAB1796) at dilution of 1:100 (40x lens).

Immunofluorescence - [KO Validated] CSNK1E Polyclonal Antibody (CAB1796)

Immunofluorescence analysis of HeLa cells using CSNK1E antibody (CAB1796). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Application:
IP
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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