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[KO Validated] CSK Polyclonal Antibody (CAB0735)

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CAB0735
  • Western blot - [KO Validated] CSK Polyclonal Antibody (CAB0735)
  • Western blot - [KO Validated] CSK Polyclonal Antibody (CAB0735)
$553.89

Description

Overview

Title:[KO Validated] CSK Polyclonal Antibody (CAB0735)
Size:100µL
Code:CAB0735
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

N/A

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human CSK (NP_001120662.1).
GeneID:1445
SwissProt:P41240
Synonyms:CSK; tyrosine-protein kinase CSK
Calculated MW:50kDa
Observed MW:51kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IF
Recommended Dilution:WB 1:500 - 1:2000; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    
Positive Samples:HL-60, A-549, Mouse spleen, Mouse liver, Rat liver
Cellular Location:Cell membrane, Cytoplasm

Antibody Images

Western blot - [KO Validated] CSK Polyclonal Antibody (CAB0735)

Western blot analysis of extracts of various cell lines, using CSK antibody (CAB0735) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.

Western blot - [KO Validated] CSK Polyclonal Antibody (CAB0735)

Western blot analysis of extracts from normal (control) and CSK knockout (KO) 293T cells, using CSK antibody (CAB0735) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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