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[KO Validated] CDK8 Polyclonal Antibody (CAB5548)

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CAB5548
  • Western blot - [KO Validated] CDK8 Polyclonal Antibody (CAB5548)
  • Western blot - [KO Validated] CDK8 Polyclonal Antibody (CAB5548)
  • Immunoprecipitation - [KO Validated] CDK8 Polyclonal Antibody (CAB5548)
$553.89

Description

Overview

Title:[KO Validated] CDK8 Polyclonal Antibody (CAB5548)
Size:100µL
Code:CAB5548
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit, cyclin C, are components of the Mediator transcriptional regulatory complex, involved in both transcriptional activation and repression by phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II. This kinase regulates transcription by targeting the cyclin-dependent kinase 7 subunits of the general transcription initiation factor IIH, thus providing a link between the Mediator complex and the basal transcription machinery. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 365-464 of human CDK8 (NP_001251.1).
GeneID:1024
SwissProt:P49336
Synonyms:CDK8; K35; cyclin-dependent kinase 8
Calculated MW:53kDa
Observed MW:56kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IP
Recommended Dilution:WB 1:500 - 1:2000; IP 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunoprecipitation    
Positive Samples:Jurkat
Cellular Location:Nucleus

Antibody Images

Western blot - [KO Validated] CDK8 Polyclonal Antibody (CAB5548)

Western blot analysis of extracts of Jurkat cells, using CDK8 antibody (CAB5548) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Western blot - [KO Validated] CDK8 Polyclonal Antibody (CAB5548)

Western blot analysis of extracts from normal (control) and CDK8 knockout (KO) HeLa cells, using CDK8 antibody (CAB5548) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Immunoprecipitation - [KO Validated] CDK8 Polyclonal Antibody (CAB5548)

Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug CDK8 antibody (CAB5548). Western blot was performed from the immunoprecipitate using CDK8 antibody (CAB5548) at a dilition of 1:1000.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IP
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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