[KO Validated] CDK6 Polyclonal Antibody (CAB1545)

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CAB1545
  • Western blot - [KO Validated] CDK6 Polyclonal Antibody (CAB1545)
  • Immunoprecipitation - [KO Validated] CDK6 Polyclonal Antibody (CAB1545)
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Description

Antibody DatasheetAntibody MSDS

[KO Validated] CDK6 Polyclonal Antibody (CAB1545)

Overview

Title:[KO Validated] CDK6 Polyclonal Antibody (CAB1545)
Size:100µL
Code:CAB1545
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Expression of this gene is up-regulated in some types of cancer. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-326 of human CDK6 (NP_001250.1).
GeneID:1021
SwissProt:Q00534
Synonyms:CDK6; MCPH12; PLSTIRE; cyclin-dependent kinase 6
Calculated MW:36kDa
Observed MW:37kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF; IP
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200; IP 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive Samples:HeLa, Jurkat, K-562
Cellular Location:Cell projection, Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center, ruffle

Antibody Images

Western blot - [KO Validated] CDK6 Polyclonal Antibody (CAB1545)

Western blot analysis of extracts from normal (control) and CDK6 knockout (KO) HeLa cells, using CDK6 antibody (CAB1545) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

Immunoprecipitation - [KO Validated] CDK6 Polyclonal Antibody (CAB1545)

Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug CDK6 antibody (CAB1545). Western blot was performed from the immunoprecipitate using CDK6 antibody (CAB1545) at a dilition of 1:1000.

[KO Validated] CDK6 Polyclonal Antibody (CAB1545) Protein Information

UniProt Protein Function:CDK6: a protein kinase of the CDK family. Important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. Phosphorylates and regulates the activity of the Rb tumor suppressor protein. Overexpressed and/or disrupted by translocation in leukemias, lymphomas and other cancers and amplified in gliomas and rodent cancers.
UniProt Protein Details:

Protein type:Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.22; Protein kinase, CMGC; Cell cycle regulation; CMGC group; CDK family; CDK/CDK4 subfamily; CDK4 subfamily

Chromosomal Location of Human Ortholog: 7q21-q22

Cellular Component: centrosome; cyclin-dependent protein kinase holoenzyme complex; cytoplasm; cytosol; nucleoplasm; nucleus; ruffle

Molecular Function:ATP binding; cyclin binding; cyclin-dependent protein kinase activity; protein binding

Biological Process: astrocyte development; cell cycle arrest; cell dedifferentiation; dentate gyrus development; G1/S transition of mitotic cell cycle; generation of neurons; gliogenesis; lateral ventricle development; negative regulation of cell cycle; negative regulation of cell differentiation; negative regulation of cell proliferation; negative regulation of epithelial cell proliferation; negative regulation of myeloid cell differentiation; negative regulation of osteoblast differentiation; positive regulation of cell-matrix adhesion; positive regulation of fibroblast proliferation; protein amino acid phosphorylation; regulation of erythrocyte differentiation; regulation of gene expression; response to virus

Disease: Microcephaly 12, Primary, Autosomal Recessive

NCBI Summary:The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Expression of this gene is up-regulated in some types of cancer. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2009]
UniProt Code:Q00534
NCBI GenInfo Identifier:223718134
NCBI Gene ID:1021
NCBI Accession:NP_001138778.1
UniProt Secondary Accession:Q00534,A4D1G0,
UniProt Related Accession:Q00534
Molecular Weight:36,938 Da
NCBI Full Name:cyclin-dependent kinase 6
NCBI Synonym Full Names:cyclin dependent kinase 6
NCBI Official Symbol:CDK6  
NCBI Official Synonym Symbols:MCPH12; PLSTIRE  
NCBI Protein Information:cyclin-dependent kinase 6
UniProt Protein Name:Cyclin-dependent kinase 6
UniProt Synonym Protein Names:Cell division protein kinase 6; Serine/threonine-protein kinase PLSTIRE
Protein Family:Cyclin-dependent kinase
UniProt Gene Name:CDK6  
UniProt Entry Name:CDK6_HUMAN
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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Application:
IP
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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