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[KO Validated] CDK6 Polyclonal Antibody (CAB1545)

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CAB1545
  • Western blot - [KO Validated] CDK6 Polyclonal Antibody (CAB1545)
  • Immunoprecipitation - [KO Validated] CDK6 Polyclonal Antibody (CAB1545)
$553.89

Description

Overview

Title:[KO Validated] CDK6 Polyclonal Antibody (CAB1545)
Size:100µL
Code:CAB1545
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Expression of this gene is up-regulated in some types of cancer. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-326 of human CDK6 (NP_001250.1).
GeneID:1021
SwissProt:Q00534
Synonyms:CDK6; MCPH12; PLSTIRE; cyclin-dependent kinase 6
Calculated MW:36kDa
Observed MW:37kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF; IP
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200; IP 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive Samples:HeLa, Jurkat, K-562
Cellular Location:Cell projection, Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center, ruffle

Antibody Images

Western blot - [KO Validated] CDK6 Polyclonal Antibody (CAB1545)

Western blot analysis of extracts from normal (control) and CDK6 knockout (KO) HeLa cells, using CDK6 antibody (CAB1545) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

Immunoprecipitation - [KO Validated] CDK6 Polyclonal Antibody (CAB1545)

Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug CDK6 antibody (CAB1545). Western blot was performed from the immunoprecipitate using CDK6 antibody (CAB1545) at a dilition of 1:1000.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Application:
IP
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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