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[KO Validated] CD44 Polyclonal Antibody (CAB12410)

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CAB12410
  • Western blot - [KO Validated] CD44 Polyclonal Antibody (CAB12410)
  • Western blot - [KO Validated] CD44 Polyclonal Antibody (CAB12410)
  • Immunohistochemistry - [KO Validated] CD44 Polyclonal Antibody (CAB12410)
  • Immunofluorescence - [KO Validated] CD44 Polyclonal Antibody (CAB12410)
$553.89

Description

Overview

Title:[KO Validated] CD44 Polyclonal Antibody (CAB12410)
Size:100µL
Code:CAB12410
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

Immunogen Information

Immunogen:Recombinant protein of human CD44
GeneID:960
SwissProt:P16070
Synonyms:CD44; CDW44; CSPG8; ECMR-III; HCELL; HUTCH-I; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CD44 antigen
Calculated MW:3kDa/15kDa/32-53kDa/73-81kDa
Observed MW:82kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:Mouse spleen
Cellular Location:Cell membrane, Single-pass type I membrane protein

Antibody Images

Western blot - [KO Validated] CD44 Polyclonal Antibody (CAB12410)

Western blot analysis of extracts of mouse spleen, using CD44 Antibody (CAB12410) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Western blot - [KO Validated] CD44 Polyclonal Antibody (CAB12410)

Western blot analysis of extracts from normal (control) and CD44 knockout (KO) HeLa cells, using CD44 antibody (CAB12410) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

Immunohistochemistry - [KO Validated] CD44 Polyclonal Antibody (CAB12410)

Immunohistochemistry of paraffin-embedded rat bronchus using CD44 antibody (CAB12410) at dilution of 1:100 (40x lens).

Immunofluorescence - [KO Validated] CD44 Polyclonal Antibody (CAB12410)

Immunofluorescence analysis of HeLa cells using CD44 antibody (CAB12410) at dilution of 1:100. Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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