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[KO Validated] CCND3 Polyclonal Antibody (CAB0746)

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CAB0746
  • Western blot - [KO Validated] CCND3 Polyclonal Antibody (CAB0746)
  • Western blot - [KO Validated] CCND3 Polyclonal Antibody (CAB0746)
$553.89

Description

Overview

Title:[KO Validated] CCND3 Polyclonal Antibody (CAB0746)
Size:100µL
Code:CAB0746
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 201-292 of human CCND3 (NP_001751.1).
GeneID:896
SwissProt:P30281
Synonyms:CCND3; cyclin D3
Calculated MW:9kDa/22kDa/24kDa/32kDa
Observed MW:36kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:HeLa, K-562, Mouse spleen
Cellular Location:Cytoplasm, Membrane, Nucleus

Antibody Images

Western blot - [KO Validated] CCND3 Polyclonal Antibody (CAB0746)

Western blot analysis of extracts of various cell lines, using CCND3 antibody (CAB0746) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Western blot - [KO Validated] CCND3 Polyclonal Antibody (CAB0746)

Western blot analysis of extracts from normal (control) and CCND3 knockout (KO) HeLa cells, using CCND3 antibody (CAB0746) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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