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[KO Validated] CBX3 Polyclonal Antibody (CAB2248)

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CAB2248
  • Western blot - [KO Validated] CBX3 Polyclonal Antibody (CAB2248)
  • Western blot - [KO Validated] CBX3 Polyclonal Antibody (CAB2248)
  • Western blot - [KO Validated] CBX3 Polyclonal Antibody (CAB2248)
  • Immunohistochemistry - [KO Validated] CBX3 Polyclonal Antibody (CAB2248)
  • Immunohistochemistry - [KO Validated] CBX3 Polyclonal Antibody (CAB2248)
  • Immunofluorescence - [KO Validated] CBX3 Polyclonal Antibody (CAB2248)
€499.00

Description

Overview

Title:[KO Validated] CBX3 Polyclonal Antibody (CAB2248)
Size:100µL
Code:CAB2248
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human CBX3 (NP_009207.2).
GeneID:11335
SwissProt:Q13185
Synonyms:CBX3; HECH; HP1-GAMMA; HP1Hs-gamma; chromobox 3
Calculated MW:20kDa
Observed MW:23kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:HeLa, MCF-7, 293T, A-549, NCI-H460, Mouse brain, Mouse lung, Mouse spleen
Cellular Location:Nucleus

Antibody Images

Western blot - [KO Validated] CBX3 Polyclonal Antibody (CAB2248)

Western blot analysis of extracts of various cell lines, using CBX3 antibody (CAB2248) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

Western blot - [KO Validated] CBX3 Polyclonal Antibody (CAB2248)

Western blot analysis of extracts of various cells, using CBX3 antibody (CAB2248) at 1:1000 dilution ratio through one-step method.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

Western blot - [KO Validated] CBX3 Polyclonal Antibody (CAB2248)

Western blot analysis of extracts from normal (control) and CBX3 knockout (KO) HeLa cells, using CBX3 antibody (CAB2248) at 1:500 dilution.

Immunohistochemistry - [KO Validated] CBX3 Polyclonal Antibody (CAB2248)

Immunohistochemistry of paraffin-embedded human lung cancer using CBX3 antibody (CAB2248) at dilution of 1:200 (40x lens).

Immunohistochemistry - [KO Validated] CBX3 Polyclonal Antibody (CAB2248)

Immunohistochemistry of paraffin-embedded human gastric using CBX3 antibody (CAB2248) at dilution of 1:200 (40x lens).

Immunofluorescence - [KO Validated] CBX3 Polyclonal Antibody (CAB2248)

Immunofluorescence analysis of U2OS cells using CBX3 antibody (CAB2248) at dilution of 1:100. Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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