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[KO Validated] CAST Polyclonal Antibody (CAB7634)

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CAB7634
  • Western blot - [KO Validated] CAST Polyclonal Antibody (CAB7634)
  • Western blot - [KO Validated] CAST Polyclonal Antibody (CAB7634)
£424.15

Description

Overview

Title:[KO Validated] CAST Polyclonal Antibody (CAB7634)
Size:100µL
Code:CAB7634
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described.

Immunogen Information

Immunogen:A synthetic peptide corresponding to a sequence within amino acids 550-650 of human CAST (NP_001177371.1).
GeneID:831
SwissProt:P20810
Synonyms:CAST; BS-17; PLACK; calpastatin
Calculated MW:63kDa/71- 84kDa
Observed MW:130kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:A-549, SW480, MCF-7, HepG2, Mouse lung, Rat liver
Cellular Location:N/A

Antibody Images

Western blot - [KO Validated] CAST Polyclonal Antibody (CAB7634)

Western blot analysis of extracts of various cell lines, using CAST antibody (CAB7634) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

Western blot - [KO Validated] CAST Polyclonal Antibody (CAB7634)

Western blot analysis of extracts from normal (control) and CAST knockout (KO) HeLa cells, using CAST antibody (CAB7634) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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