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[KO Validated] CASP3 Polyclonal Antibody (CAB2156)

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CAB2156
  • Western blot - [KO Validated] CASP3 Polyclonal Antibody (CAB2156)
  • Western blot - [KO Validated] CASP3 Polyclonal Antibody (CAB2156)
  • Immunohistochemistry - [KO Validated] CASP3 Polyclonal Antibody (CAB2156)
  • Immunohistochemistry - [KO Validated] CASP3 Polyclonal Antibody (CAB2156)
  • Immunohistochemistry - [KO Validated] CASP3 Polyclonal Antibody (CAB2156)
  • Immunohistochemistry - [KO Validated] CASP3 Polyclonal Antibody (CAB2156)
  • Immunohistochemistry - [KO Validated] CASP3 Polyclonal Antibody (CAB2156)
  • Immunofluorescence - [KO Validated] CASP3 Polyclonal Antibody (CAB2156)
  • Immunofluorescence - [KO Validated] CASP3 Polyclonal Antibody (CAB2156)
  • Immunofluorescence - [KO Validated] CASP3 Polyclonal Antibody (CAB2156)
£254.15

Description

Overview

Title:[KO Validated] CASP3 Polyclonal Antibody (CAB2156)
Size:100µL
Code:CAB2156
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 55-160 of human CASP3 (NP_004337.2).
GeneID:836
SwissProt:P42574
Synonyms:CASP3; CPP32; CPP32B; SCA-1; caspase-3
Calculated MW:31kDa
Observed MW:17kDa, 35kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:Jurkat
Cellular Location:Cytoplasm

Antibody Images

Western blot - [KO Validated] CASP3 Polyclonal Antibody (CAB2156)

Western blot analysis of extracts of Jurkat cells, using CASP3 antibody (CAB2156) at 1:1000 dilution. Jurkat cells treated by Etoposide 25uM etoposide for 5 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.

Western blot - [KO Validated] CASP3 Polyclonal Antibody (CAB2156)

Western blot analysis of extracts from normal (control) and CASP3 knockout (KO) 293T cells, using CASP3 antibody (CAB2156) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.

Immunohistochemistry - [KO Validated] CASP3 Polyclonal Antibody (CAB2156)

Immunohistochemistry of paraffin-embedded rat brain using CASP3 antibody (CAB2156) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] CASP3 Polyclonal Antibody (CAB2156)

Immunohistochemistry of paraffin-embedded rat spleen using CASP3 antibody (CAB2156) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] CASP3 Polyclonal Antibody (CAB2156)

Immunohistochemistry of paraffin-embedded human tonsil using CASP3 antibody (CAB2156) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] CASP3 Polyclonal Antibody (CAB2156)

Immunohistochemistry of paraffin-embedded human placenta using CASP3 antibody (CAB2156) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] CASP3 Polyclonal Antibody (CAB2156)

Immunohistochemistry of paraffin-embedded human gastric cancer using CASP3 antibody (CAB2156) at dilution of 1:100 (40x lens).

Immunofluorescence - [KO Validated] CASP3 Polyclonal Antibody (CAB2156)

Immunofluorescence analysis of C6 cells using CASP3 antibody (CAB2156) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence - [KO Validated] CASP3 Polyclonal Antibody (CAB2156)

Immunofluorescence analysis of HeLa cells using CASP3 antibody (CAB2156) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence - [KO Validated] CASP3 Polyclonal Antibody (CAB2156)

Immunofluorescence analysis of NIH/3T3 cells using CASP3 antibody (CAB2156) at dilution of 1:100. Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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