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[KO Validated] CAPN2 Polyclonal Antibody (CAB1861)

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CAB1861
  • Western blot - [KO Validated] CAPN2 Polyclonal Antibody (CAB1861)
  • Western blot - [KO Validated] CAPN2 Polyclonal Antibody (CAB1861)
  • Immunofluorescence - [KO Validated] CAPN2 Polyclonal Antibody (CAB1861)
€499.00

Description

Overview

Title:[KO Validated] CAPN2 Polyclonal Antibody (CAB1861)
Size:100µL
Code:CAB1861
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 350-680 of human CAPN2 (NP_001739.2).
GeneID:824
SwissProt:P17655
Synonyms:CAPN2; CANP2; CANPL2; CANPml; mCANP; calpain 2
Calculated MW:71kDa/79kDa
Observed MW:80kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IF
Recommended Dilution:WB 1:500 - 1:2000; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    
Positive Samples:H460, A-549, HeLa, Mouse kidney, Mouse lung
Cellular Location:Cell membrane, Cytoplasm

Antibody Images

Western blot - [KO Validated] CAPN2 Polyclonal Antibody (CAB1861)

Western blot analysis of extracts of various cell lines, using CAPN2 antibody (CAB1861) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Western blot - [KO Validated] CAPN2 Polyclonal Antibody (CAB1861)

Western blot analysis of extracts from normal (control) and CAPN2 knockout (KO) 293T cells, using CAPN2 antibody (CAB1861) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Immunofluorescence - [KO Validated] CAPN2 Polyclonal Antibody (CAB1861)

Immunofluorescence analysis of U2OS cells using CAPN2 antibody (CAB1861) at dilution of 1:100. Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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