The cookie settings on this website are set to 'allow all cookies' to give you the very best experience. Please click Accept Cookies to continue to use the site.

[KO Validated] CAPG Polyclonal Antibody (CAB7324)

(No reviews yet) Write a Review
SKU:
CAB7324
  • Western blot - [KO Validated] CAPG Polyclonal Antibody (CAB7324)
  • Western blot - [KO Validated] CAPG Polyclonal Antibody (CAB7324)
  • Western blot - [KO Validated] CAPG Polyclonal Antibody (CAB7324)
  • Immunohistochemistry - [KO Validated] CAPG Polyclonal Antibody (CAB7324)
  • Immunohistochemistry - [KO Validated] CAPG Polyclonal Antibody (CAB7324)
£424.15

Description

Overview

Title:[KO Validated] CAPG Polyclonal Antibody (CAB7324)
Size:100µL
Code:CAB7324
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a member of the gelsolin/villin family of actin-regulatory proteins. The encoded protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. By capping the barbed ends of actin filaments, the encoded protein contributes to the control of actin-based motility in non-muscle cells. Alternatively spliced transcript variants have been observed for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-348 of human CAPG (NP_001738.2).
GeneID:822
SwissProt:P40121
Synonyms:CAPG; AFCP; HEL-S-66; MCP; macrophage-capping protein
Calculated MW:36kDa/38kDa
Observed MW:38kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:THP-1, HL-60, Mouse lung, Mouse spleen, Rat heart, Rat lung
Cellular Location:Cytoplasm, Melanosome, Nucleus

Antibody Images

Western blot - [KO Validated] CAPG Polyclonal Antibody (CAB7324)

Western blot analysis of extracts of various cell lines, using CAPG Antibody (CAB7324) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Western blot - [KO Validated] CAPG Polyclonal Antibody (CAB7324)

Western blot analysis of extracts of various cell lines, using CAPG Antibody (CAB7324) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Western blot - [KO Validated] CAPG Polyclonal Antibody (CAB7324)

Western blot analysis of extracts from normal (control) and CAPG knockout (KO) HeLa cells, using CAPG antibody (CAB7324) at 1:1000 dilution.

Immunohistochemistry - [KO Validated] CAPG Polyclonal Antibody (CAB7324)

Immunohistochemistry of paraffin-embedded human lung cancer using CAPG Antibody (CAB7324) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] CAPG Polyclonal Antibody (CAB7324)

Immunohistochemistry of paraffin-embedded human liver cancer using CAPG Antibody (CAB7324) at dilution of 1:100 (40x lens).

Datasheets

Antibody DatasheetAntibody MSDS

View AllClose

Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
View AllClose