[KO Validated] BCL10 Polyclonal Antibody (CAB1106)

(No reviews yet) Write a Review
SKU:
CAB1106
  • Western blot - [KO Validated] BCL10 Polyclonal Antibody (CAB1106)
  • Western blot - [KO Validated] BCL10 Polyclonal Antibody (CAB1106)
€499.00
Frequently bought together:

Description

Antibody DatasheetAntibody MSDS

[KO Validated] BCL10 Polyclonal Antibody (CAB1106)

Overview

Title:[KO Validated] BCL10 Polyclonal Antibody (CAB1106)
Size:100µL
Code:CAB1106
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene was identified by its translocation in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. The protein encoded by this gene contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. This protein is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy. Alternative splicing results in multiple transcript variants.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-233 of human BCL10 (NP_003912.1).
GeneID:8915
SwissProt:O95999
Synonyms:BCL10; CARMEN; CIPER; CLAP; IMD37; c-E10; mE10; B-cell CLL/lymphoma 10
Calculated MW:26kDa
Observed MW:33kDa

Applications

Reactivity:Human
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:MCF-7, DU145, A-549, BT-474, Jurkat
Cellular Location:Cytoplasm, Membrane raft, perinuclear region

Antibody Images

Western blot - [KO Validated] BCL10 Polyclonal Antibody (CAB1106)

Western blot analysis of extracts of various cell lines, using BCL10 antibody (CAB1106) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Western blot - [KO Validated] BCL10 Polyclonal Antibody (CAB1106)

Western blot analysis of extracts from normal (control) and BCL10 knockout (KO) HeLa cells, using BCL10 antibody (CAB1106) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

[KO Validated] BCL10 Polyclonal Antibody (CAB1106) Protein Information

UniProt Protein Function:Bcl-10: a ubiquitous pro-apoptotic protein that promotes pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Self-associates via CARD-CARD interactions; forms a tight complex with MALT1. Interacts with other CARD-proteins such as CARD9, CARD10, CARD11 and CARD14. Binds caspase-9 with its C-terminal domain. Interacts with TRAF2 and BIRC2/c-IAP2. Defects in BCL10 are involved in various types of cancer.
UniProt Protein Details:

Protein type:Apoptosis; Tumor suppressor; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 1p22

Cellular Component: CBM complex; protein complex; lysosome; immunological synapse; cytosol; lipid raft; T cell receptor complex; cytoplasmic microtubule; perinuclear region of cytoplasm; cytoplasm; plasma membrane; lipopolysaccharide receptor complex; nucleus

Molecular Function:protein C-terminus binding; protein kinase B binding; ubiquitin binding; protease binding; protein self-association; transcription coactivator activity; transcription factor binding; protein kinase binding; NF-kappaB binding; protein binding; enzyme binding; ubiquitin protein ligase binding; kinase activator activity; kinase binding

Biological Process: cell death; response to fungus; positive regulation of transcription, DNA-dependent; positive regulation of caspase activity; B cell apoptosis; T cell receptor signaling pathway; interleukin-6 biosynthetic process; activation of NF-kappaB transcription factor; negative regulation of mature B cell apoptosis; response to molecule of bacterial origin; protein homooligomerization; response to food; adaptive immune response; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-8 biosynthetic process; protein oligomerization; positive regulation of protein ubiquitination; lymphotoxin A biosynthetic process; regulation of T cell receptor signaling pathway; neural tube closure; toll-like receptor signaling pathway; innate immune response; cellular defense response; positive regulation of mast cell cytokine production; positive regulation of T cell activation; immunoglobulin mediated immune response; positive regulation of phosphorylation

Disease: Follicular Lymphoma, Susceptibility To, 1; Mesothelioma, Malignant; Gastric Lymphoma, Primary; Testicular Germ Cell Tumor; Immunodeficiency 37

NCBI Summary:This gene was identified by its translocation in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. The protein encoded by this gene contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. This protein is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy. [provided by RefSeq, Jul 2008]
UniProt Code:O95999
NCBI GenInfo Identifier:4502379
NCBI Gene ID:8915
NCBI Accession:NP_003912.1
UniProt Secondary Accession:O95999,Q5VUF1,
UniProt Related Accession:O95999
Molecular Weight:26,252 Da
NCBI Full Name:B-cell lymphoma/leukemia 10
NCBI Synonym Full Names:B-cell CLL/lymphoma 10
NCBI Official Symbol:BCL10  
NCBI Official Synonym Symbols:CLAP; mE10; CIPER; c-E10; CARMEN  
NCBI Protein Information:B-cell lymphoma/leukemia 10; hCLAP; cCARMEN; cellular-E10; cellular homolog of vCARMEN; CARD-containing proapoptotic protein; CARD containing molecule enhancing NF-kB; CARD-containing apoptotic signaling protein; caspase-recruiting domain-containing prote
UniProt Protein Name:B-cell lymphoma/leukemia 10
UniProt Synonym Protein Names:B-cell CLL/lymphoma 10; Bcl-10; CARD-containing molecule enhancing NF-kappa-B; CARD-like apoptotic protein; hCLAP; CED-3/ICH-1 prodomain homologous E10-like regulator; CIPER; Cellular homolog of vCARMEN; cCARMEN; Cellular-E10; c-E10; Mammalian CARD-containing adapter molecule E10
Protein Family:B-cell lymphoma/leukemia
UniProt Gene Name:BCL10  
UniProt Entry Name:BCL10_HUMAN
View AllClose

Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
View AllClose