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[KO Validated] BCL10 Polyclonal Antibody (CAB1106)

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CAB1106
  • Western blot - [KO Validated] BCL10 Polyclonal Antibody (CAB1106)
  • Western blot - [KO Validated] BCL10 Polyclonal Antibody (CAB1106)
$553.89

Description

Overview

Title:[KO Validated] BCL10 Polyclonal Antibody (CAB1106)
Size:100µL
Code:CAB1106
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene was identified by its translocation in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. The protein encoded by this gene contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. This protein is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy. Alternative splicing results in multiple transcript variants.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-233 of human BCL10 (NP_003912.1).
GeneID:8915
SwissProt:O95999
Synonyms:BCL10; CARMEN; CIPER; CLAP; IMD37; c-E10; mE10; B-cell CLL/lymphoma 10
Calculated MW:26kDa
Observed MW:33kDa

Applications

Reactivity:Human
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:MCF-7, DU145, A-549, BT-474, Jurkat
Cellular Location:Cytoplasm, Membrane raft, perinuclear region

Antibody Images

Western blot - [KO Validated] BCL10 Polyclonal Antibody (CAB1106)

Western blot analysis of extracts of various cell lines, using BCL10 antibody (CAB1106) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Western blot - [KO Validated] BCL10 Polyclonal Antibody (CAB1106)

Western blot analysis of extracts from normal (control) and BCL10 knockout (KO) HeLa cells, using BCL10 antibody (CAB1106) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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