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[KO Validated] BAZ1B Polyclonal Antibody (CAB9851)

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CAB9851
  • Western blot - [KO Validated] BAZ1B Polyclonal Antibody (CAB9851)
  • Western blot - [KO Validated] BAZ1B Polyclonal Antibody (CAB9851)
  • Immunohistochemistry - [KO Validated] BAZ1B Polyclonal Antibody (CAB9851)
  • Immunohistochemistry - [KO Validated] BAZ1B Polyclonal Antibody (CAB9851)
  • Immunohistochemistry - [KO Validated] BAZ1B Polyclonal Antibody (CAB9851)
  • Immunohistochemistry - [KO Validated] BAZ1B Polyclonal Antibody (CAB9851)
  • Immunohistochemistry - [KO Validated] BAZ1B Polyclonal Antibody (CAB9851)
  • Immunofluorescence - [KO Validated] BAZ1B Polyclonal Antibody (CAB9851)
$553.89

Description

Overview

Title:[KO Validated] BAZ1B Polyclonal Antibody (CAB9851)
Size:100µL
Code:CAB9851
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a member of the bromodomain protein family. The bromodomain is a structural motif characteristic of proteins involved in chromatin-dependent regulation of transcription. This gene is deleted in Williams-Beuren syndrome, a developmental disorder caused by deletion of multiple genes at 7q11.23.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human BAZ1B (NP_115784.1).
GeneID:9031
SwissProt:Q9UIG0
Synonyms:BAZ1B; WBSCR10; WBSCR9; WSTF; tyrosine-protein kinase BAZ1B
Calculated MW:170kDa
Observed MW:190kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:SW480, BT-474, SKOV3, HeLa, HepG2, Mouse thymus
Cellular Location:Nucleus

Antibody Images

Western blot - [KO Validated] BAZ1B Polyclonal Antibody (CAB9851)

Western blot analysis of extracts of various cell lines, using BAZ1B antibody (CAB9851) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

Western blot - [KO Validated] BAZ1B Polyclonal Antibody (CAB9851)

Western blot analysis of extracts from normal (control) and BAZ1B knockout (KO) HeLa cells, using BAZ1B antibody (CAB9851) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Immunohistochemistry - [KO Validated] BAZ1B Polyclonal Antibody (CAB9851)

Immunohistochemistry of paraffin-embedded rat brain using BAZ1B antibody (CAB9851) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] BAZ1B Polyclonal Antibody (CAB9851)

Immunohistochemistry of paraffin-embedded mouse lung using BAZ1B antibody (CAB9851) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] BAZ1B Polyclonal Antibody (CAB9851)

Immunohistochemistry of paraffin-embedded mouse brain using BAZ1B antibody (CAB9851) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] BAZ1B Polyclonal Antibody (CAB9851)

Immunohistochemistry of paraffin-embedded mouse stomach using BAZ1B antibody (CAB9851) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] BAZ1B Polyclonal Antibody (CAB9851)

Immunohistochemistry of paraffin-embedded mouse heart using BAZ1B antibody (CAB9851) at dilution of 1:100 (40x lens).

Immunofluorescence - [KO Validated] BAZ1B Polyclonal Antibody (CAB9851)

Immunofluorescence analysis of HeLa cells using BAZ1B antibody (CAB9851) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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