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[KO Validated] B2M Polyclonal Antibody (CAB12404)

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CAB12404
  • Western blot - [KO Validated] B2M Polyclonal Antibody (CAB12404)
  • Western blot - [KO Validated] B2M Polyclonal Antibody (CAB12404)
  • Immunohistochemistry - [KO Validated] B2M Polyclonal Antibody (CAB12404)
  • Immunohistochemistry - [KO Validated] B2M Polyclonal Antibody (CAB12404)
$553.89

Description

Overview

Title:[KO Validated] B2M Polyclonal Antibody (CAB12404)
Size:100µL
Code:CAB12404
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 21-119 of human B2M (NP_004039.1).
GeneID:567
SwissProt:P61769
Synonyms:B2M; IMD43; beta-2-microglobulin
Calculated MW:13kDa
Observed MW:12kDa

Applications

Reactivity:Human, Mouse, Rat, Monkey
Tested Applications:WB; IHC; IP
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IP 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunoprecipitation    
Positive Samples:Jurkat, COS7, HeLa, K-562, Mouse liver, Mouse spleen
Cellular Location:Secreted

Antibody Images

Western blot - [KO Validated] B2M Polyclonal Antibody (CAB12404)

Western blot analysis of extracts of various cell lines, using B2M antibody (CAB12404) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).

Western blot - [KO Validated] B2M Polyclonal Antibody (CAB12404)

Western blot analysis of extracts from normal (control) and B2M knockout (KO) 293T cells, using B2M antibody (CAB12404) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Immunohistochemistry - [KO Validated] B2M Polyclonal Antibody (CAB12404)

Immunohistochemistry of paraffin-embedded rat brain using B2M antibody (CAB12404) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] B2M Polyclonal Antibody (CAB12404)

Immunohistochemistry of paraffin-embedded human prostate using B2M antibody (CAB12404) at dilution of 1:100 (40x lens).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IP
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Reactivity:
Monkey
Host Species:
Rabbit
Isotype:
IgG
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