The cookie settings on this website are set to 'allow all cookies' to give you the very best experience. Please click Accept Cookies to continue to use the site.

[KO Validated] ARHGEF1 Polyclonal Antibody (CAB4274)

(No reviews yet) Write a Review
SKU:
CAB4274
  • Western blot - [KO Validated] ARHGEF1 Polyclonal Antibody (CAB4274)
  • Western blot - [KO Validated] ARHGEF1 Polyclonal Antibody (CAB4274)
  • Immunohistochemistry - [KO Validated] ARHGEF1 Polyclonal Antibody (CAB4274)
$553.89

Description

Overview

Title:[KO Validated] ARHGEF1 Polyclonal Antibody (CAB4274)
Size:100µL
Code:CAB4274
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 783-912 of human ARHGEF1 (NP_004697.2).
GeneID:9138
SwissProt:Q92888
Synonyms:ARHGEF1; GEF1; LBCL2; LSC; P115-RHOGEF; SUB1.5; rho guanine nucleotide exchange factor 1
Calculated MW:98kDa/102kDa/104kDa/105kDa
Observed MW:102kDa

Applications

Reactivity:Human
Tested Applications:WB; IHC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:HeLa, Jurkat, LO2
Cellular Location:Cytoplasm, Membrane

Antibody Images

Western blot - [KO Validated] ARHGEF1 Polyclonal Antibody (CAB4274)

Western blot analysis of extracts of various cell lines, using ARHGEF1 antibody (CAB4274) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Western blot - [KO Validated] ARHGEF1 Polyclonal Antibody (CAB4274)

Western blot analysis of extracts from normal (control) and ARHGEF1 knockout (KO) 293T cells, using ARHGEF1 antibody (CAB4274) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

Immunohistochemistry - [KO Validated] ARHGEF1 Polyclonal Antibody (CAB4274)

Immunohistochemistry of paraffin-embedded human tonsil using ARHGEF1 antibody (CAB4274) at dilution of 1:100 (40x lens).

Datasheets

Antibody DatasheetAntibody MSDS

View AllClose

Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
View AllClose