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[KO Validated] ARHGDIA Polyclonal Antibody (CAB1214)

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CAB1214
  • Western blot - [KO Validated] ARHGDIA Polyclonal Antibody (CAB1214)
  • Western blot - [KO Validated] ARHGDIA Polyclonal Antibody (CAB1214)
  • Western blot - [KO Validated] ARHGDIA Polyclonal Antibody (CAB1214)
  • Immunofluorescence - [KO Validated] ARHGDIA Polyclonal Antibody (CAB1214)
€499.00

Description

Overview

Title:[KO Validated] ARHGDIA Polyclonal Antibody (CAB1214)
Size:100µL
Code:CAB1214
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a protein that plays a key role in the regulation of signaling through Rho GTPases. The encoded protein inhibits the disassociation of Rho family members from GDP (guanine diphosphate), thereby maintaining these factors in an inactive state. Activity of this protein is important in a variety of cellular processes, and expression of this gene may be altered in tumors. Mutations in this gene have been found in individuals with nephrotic syndrome, type 8. Alternate splicing results in multiple transcript variants.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-204 of human ARHGDIA (NP_001172006.1).
GeneID:396
SwissProt:P52565
Synonyms:ARHGDIA; GDIA1; HEL-S-47e; NPHS8; RHOGDI; RHOGDI-1; rho GDP-dissociation inhibitor 1
Calculated MW:18kDa/23kDa
Observed MW:25kDa

Applications

Reactivity:Human, Mouse, Rat, Monkey
Tested Applications:WB; IF
Recommended Dilution:WB 1:500 - 1:2000; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    
Positive Samples:HeLa, COS7, PC-12, Mouse lung, Mouse spleen, Mouse kidney
Cellular Location:Cytoplasm

Antibody Images

Western blot - [KO Validated] ARHGDIA Polyclonal Antibody (CAB1214)

Western blot analysis of extracts of various cell lines, using ARHGDIA antibody (CAB1214) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Western blot - [KO Validated] ARHGDIA Polyclonal Antibody (CAB1214)

Western blot analysis of extracts of various cells, using ARHGDIA antibody (CAB1214) at 1:1000 dilution ratio through one-step method.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Western blot - [KO Validated] ARHGDIA Polyclonal Antibody (CAB1214)

Western blot analysis of extracts from normal (control) and ARHGDIA knockout (KO) 293T cells, using ARHGDIA antibody (CAB1214) at 1:1000 dilution.

Immunofluorescence - [KO Validated] ARHGDIA Polyclonal Antibody (CAB1214)

Immunofluorescence analysis of HeLa cells using ARHGDIA antibody (CAB1214). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Reactivity:
Monkey
Host Species:
Rabbit
Isotype:
IgG
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