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[KO Validated] APEX1 Polyclonal Antibody (CAB2587)

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CAB2587
  • Western blot - [KO Validated] APEX1 Polyclonal Antibody (CAB2587)
  • Western blot - [KO Validated] APEX1 Polyclonal Antibody (CAB2587)
  • Immunofluorescence - [KO Validated] APEX1 Polyclonal Antibody (CAB2587)
  • Immunofluorescence - [KO Validated] APEX1 Polyclonal Antibody (CAB2587)
  • Immunoprecipitation - [KO Validated] APEX1 Polyclonal Antibody (CAB2587)
$553.89

Description

Overview

Title:[KO Validated] APEX1 Polyclonal Antibody (CAB2587)
Size:100µL
Code:CAB2587
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-318 of human APEX1 (NP_542380.1).
GeneID:328
SwissProt:P27695
Synonyms:APEX1; APE; APE1; APEN; APEX; APX; HAP1; REF1; DNA-(apurinic or apyrimidinic site) lyase
Calculated MW:35kDa
Observed MW:39kDa

Applications

Reactivity:Human
Tested Applications:WB; IF; IP
Recommended Dilution:WB 1:500 - 1:2000; IF 1:50 - 1:100; IP 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    Immunoprecipitation    
Positive Samples:HeLa, HepG2
Cellular Location:Cytoplasm, Endoplasmic reticulum, Mitochondrion, Nucleus, Nucleus speckle, nucleolus

Antibody Images

Western blot - [KO Validated] APEX1 Polyclonal Antibody (CAB2587)

Western blot analysis of extracts of various cell lines, using APEX1 antibody (CAB2587).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Western blot - [KO Validated] APEX1 Polyclonal Antibody (CAB2587)

Western blot analysis of extracts from normal (control) and APEX1 knockout (KO) 293T cells, using APEX1 antibody (CAB2587) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Immunofluorescence - [KO Validated] APEX1 Polyclonal Antibody (CAB2587)

Immunofluorescence analysis of HeLa cells using APEX1 antibody (CAB2587). Blue: DAPI for nuclear staining.

Immunofluorescence - [KO Validated] APEX1 Polyclonal Antibody (CAB2587)

Immunofluorescence analysis of A549 cells using APEX1 antibody (CAB2587).

Immunoprecipitation - [KO Validated] APEX1 Polyclonal Antibody (CAB2587)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug APEX1 antibody (CAB2587). Western blot was performed from the immunoprecipitate using APEX1 antibody (CAB2587) at a dilition of 1:1000.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IF
Application:
IP
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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