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[KO Validated] ANXA5 Polyclonal Antibody (CAB1728)

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CAB1728
  • Western blot - [KO Validated] ANXA5 Polyclonal Antibody (CAB1728)
  • Western blot - [KO Validated] ANXA5 Polyclonal Antibody (CAB1728)
$553.89

Description

Overview

Title:[KO Validated] ANXA5 Polyclonal Antibody (CAB1728)
Size:100µL
Code:CAB1728
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human ANXA5 (NP_001145.1).
GeneID:308
SwissProt:P08758
Synonyms:ANXA5; ANX5; ENX2; HEL-S-7; PP4; RPRGL3; annexin A5
Calculated MW:35kDa
Observed MW:35kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:HepG2, HeLa, DU145, PC-3, Mouse lung
Cellular Location:N/A

Antibody Images

Western blot - [KO Validated] ANXA5 Polyclonal Antibody (CAB1728)

Western blot analysis of extracts of various cell lines, using ANXA5 antibody (CAB1728) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Western blot - [KO Validated] ANXA5 Polyclonal Antibody (CAB1728)

Western blot analysis of extracts from normal (control) and ANXA5 knockout (KO) 293T cells, using ANXA5 antibody (CAB1728) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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