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[KO Validated] ANXA2 Polyclonal Antibody (CAB12397)

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CAB12397
  • Western blot - [KO Validated] ANXA2 Polyclonal Antibody (CAB12397)
  • Western blot - [KO Validated] ANXA2 Polyclonal Antibody (CAB12397)
  • Immunohistochemistry - [KO Validated] ANXA2 Polyclonal Antibody (CAB12397)
  • Immunohistochemistry - [KO Validated] ANXA2 Polyclonal Antibody (CAB12397)
  • Immunohistochemistry - [KO Validated] ANXA2 Polyclonal Antibody (CAB12397)
  • Immunofluorescence - [KO Validated] ANXA2 Polyclonal Antibody (CAB12397)
$553.89

Description

Overview

Title:[KO Validated] ANXA2 Polyclonal Antibody (CAB12397)
Size:100µL
Code:CAB12397
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-339 of human ANXA2 (NP_004030.1).
GeneID:302
SwissProt:P07355
Synonyms:ANXA2; ANX2; ANX2L4; CAL1H; HEL-S-270; LIP2; LPC2; LPC2D; P36; PAP-IV; annexin A2
Calculated MW:38kDa/40kDa
Observed MW:38kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:20 - 1:50
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:SH-SY5Y, HepG2, Mouse lung
Cellular Location:Melanosome, Secreted, basement membrane, extracellular matrix, extracellular space

Antibody Images

Western blot - [KO Validated] ANXA2 Polyclonal Antibody (CAB12397)

Western blot analysis of extracts of various cell lines, using ANXA2 Antibody (CAB12397) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Western blot - [KO Validated] ANXA2 Polyclonal Antibody (CAB12397)

Western blot analysis of extracts from normal (control) and ANXA2 knockout (KO) HeLa cells, using ANXA2 antibody (CAB12397) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.

Immunohistochemistry - [KO Validated] ANXA2 Polyclonal Antibody (CAB12397)

Immunohistochemistry of paraffin-embedded rat heart using ANXA2 antibody (CAB12397) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] ANXA2 Polyclonal Antibody (CAB12397)

Immunohistochemistry of paraffin-embedded mouse liver using ANXA2 antibody (CAB12397) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] ANXA2 Polyclonal Antibody (CAB12397)

Immunohistochemistry of paraffin-embedded mouse heart using ANXA2 antibody (CAB12397) at dilution of 1:100 (40x lens).

Immunofluorescence - [KO Validated] ANXA2 Polyclonal Antibody (CAB12397)

Immunofluorescence analysis of A549 cells using ANXA2 antibody (CAB12397). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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