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[KO Validated] ANXA1 Polyclonal Antibody (CAB1118)

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CAB1118
  • Western blot - [KO Validated] ANXA1 Polyclonal Antibody (CAB1118)
  • Western blot - [KO Validated] ANXA1 Polyclonal Antibody (CAB1118)
  • Immunohistochemistry - [KO Validated] ANXA1 Polyclonal Antibody (CAB1118)
  • Immunohistochemistry - [KO Validated] ANXA1 Polyclonal Antibody (CAB1118)
  • Immunofluorescence - [KO Validated] ANXA1 Polyclonal Antibody (CAB1118)
$553.89

Description

Overview

Title:[KO Validated] ANXA1 Polyclonal Antibody (CAB1118)
Size:100µL
Code:CAB1118
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-346 of human ANXA1 (NP_000691.1).
GeneID:301
SwissProt:P04083
Synonyms:ANXA1; ANX1; LPC1; annexin A1
Calculated MW:38kDa
Observed MW:38kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:HeLa, NIH/3T3
Cellular Location:Apical cell membrane, Basolateral cell membrane, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoplasmic vesicle membrane, Early endosome, Endosome membrane, Extracellular side, Lateral cell membrane, Membrane, Nucleus, Peripheral membrane protein, Secreted, cilium, exosome, extracellular space, phagocytic cup, secretory vesicle lumen

Antibody Images

Western blot - [KO Validated] ANXA1 Polyclonal Antibody (CAB1118)

Western blot analysis of extracts of various cell lines, using ANXA1 antibody (CAB1118).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Western blot - [KO Validated] ANXA1 Polyclonal Antibody (CAB1118)

Western blot analysis of extracts from normal (control) and ANXA1 knockout (KO) HeLa cells, using ANXA1 antibody (CAB1118) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Immunohistochemistry - [KO Validated] ANXA1 Polyclonal Antibody (CAB1118)

Immunohistochemistry of paraffin-embedded human esophagus using ANXA1 antibody (CAB1118) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] ANXA1 Polyclonal Antibody (CAB1118)

Immunohistochemistry of paraffin-embedded mouse spleen using ANXA1 antibody (CAB1118) at dilution of 1:100 (40x lens).

Immunofluorescence - [KO Validated] ANXA1 Polyclonal Antibody (CAB1118)

Immunofluorescence analysis of HeLa cells using ANXA1 antibody (CAB1118). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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