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[KO Validated] AK2 Polyclonal Antibody (CAB6519)

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CAB6519
  • Western blot - [KO Validated] AK2 Polyclonal Antibody (CAB6519)
  • Western blot - [KO Validated] AK2 Polyclonal Antibody (CAB6519)
  • Immunohistochemistry - [KO Validated] AK2 Polyclonal Antibody (CAB6519)
$553.89

Description

Overview

Title:[KO Validated] AK2 Polyclonal Antibody (CAB6519)
Size:100µL
Code:CAB6519
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates; this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Mutations in this gene are the cause of reticular dysgenesis. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 1 and 2.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-232 of human AK2 (NP_001616.1).
GeneID:204
SwissProt:P54819
Synonyms:AK2; ADK2; adenylate kinase 2
Calculated MW:14kDa/21kDa/22kDa/24kDa/25kDa/26kDa
Observed MW:32kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:HL-60, Mouse liver, Mouse kidney, Rat liver
Cellular Location:Mitochondrion intermembrane space

Antibody Images

Western blot - [KO Validated] AK2 Polyclonal Antibody (CAB6519)

Western blot analysis of extracts of various cell lines, using AK2 antibody (CAB6519) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Western blot - [KO Validated] AK2 Polyclonal Antibody (CAB6519)

Western blot analysis of extracts from normal (control) and AK2 knockout (KO) 293T cells, using AK2 antibody (CAB6519) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Immunohistochemistry - [KO Validated] AK2 Polyclonal Antibody (CAB6519)

Immunohistochemistry of paraffin-embedded human gastric cancer using AK2 antibody (CAB6519) at dilution of 1:100 (40x lens).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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