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[KO Validated] AIFM1 Polyclonal Antibody (CAB2568)

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CAB2568
  • Western blot - [KO Validated] AIFM1 Polyclonal Antibody (CAB2568)
  • Western blot - [KO Validated] AIFM1 Polyclonal Antibody (CAB2568)
  • Immunohistochemistry - [KO Validated] AIFM1 Polyclonal Antibody (CAB2568)
  • Immunohistochemistry - [KO Validated] AIFM1 Polyclonal Antibody (CAB2568)
  • Immunohistochemistry - [KO Validated] AIFM1 Polyclonal Antibody (CAB2568)
  • Immunohistochemistry - [KO Validated] AIFM1 Polyclonal Antibody (CAB2568)
  • Immunohistochemistry - [KO Validated] AIFM1 Polyclonal Antibody (CAB2568)
  • Immunohistochemistry - [KO Validated] AIFM1 Polyclonal Antibody (CAB2568)
  • Immunohistochemistry - [KO Validated] AIFM1 Polyclonal Antibody (CAB2568)
  • Immunohistochemistry - [KO Validated] AIFM1 Polyclonal Antibody (CAB2568)
$553.89

Description

Overview

Title:[KO Validated] AIFM1 Polyclonal Antibody (CAB2568)
Size:100µL
Code:CAB2568
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6 (COXPD6), a severe mitochondrial encephalomyopathy, as well as Cowchock syndrome, also known as X-linked recessive Charcot-Marie-Tooth disease-4 (CMTX-4), a disorder resulting in neuropathy, and axonal and motor-sensory defects with deafness and mental retardation. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 334-613 of human AIFM1 (NP_004199.1).
GeneID:9131
SwissProt:O95831
Synonyms:AIFM1; AIF; CMT2D; CMTX4; COWCK; COXPD6; DFNX5; NADMR; NAMSD; PDCD8; apoptosis-inducing factor 1, mitochondrial
Calculated MW:26kDa/28kDa/35kDa/66kDa
Observed MW:67kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB; IHC; IF; IP
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200; IP 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive Samples:SW620, 22Rv1, H460, PC-3, U-937, Mouse liver, Mouse kidney, Mouse heart
Cellular Location:Cytoplasm, Mitochondrion inner membrane, Mitochondrion intermembrane space, Nucleus, perinuclear region

Antibody Images

Western blot - [KO Validated] AIFM1 Polyclonal Antibody (CAB2568)

Western blot analysis of extracts of various cell lines, using AIFM1 antibody (CAB2568) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Western blot - [KO Validated] AIFM1 Polyclonal Antibody (CAB2568)

Western blot analysis of extracts from normal (control) and AIFM1 knockout (KO) 293T cells, using AIFM1 antibody (CAB2568) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Immunohistochemistry - [KO Validated] AIFM1 Polyclonal Antibody (CAB2568)

Immunohistochemistry of paraffin-embedded human esophagus using AIFM1 Antibody (CAB2568) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] AIFM1 Polyclonal Antibody (CAB2568)

Immunohistochemistry of paraffin-embedded human esophagus using AIFM1 Antibody (CAB2568) at dilution of 1:200 (40x lens).

Immunohistochemistry - [KO Validated] AIFM1 Polyclonal Antibody (CAB2568)

Immunohistochemistry of paraffin-embedded human endometrium using AIFM1 Antibody (CAB2568) at dilution of 1:200 (40x lens).

Immunohistochemistry - [KO Validated] AIFM1 Polyclonal Antibody (CAB2568)

Immunohistochemistry of paraffin-embedded mouse esophagus using AIFM1 Antibody (CAB2568) at dilution of 1:200 (40x lens).

Immunohistochemistry - [KO Validated] AIFM1 Polyclonal Antibody (CAB2568)

Immunohistochemistry of paraffin-embedded rat lung using AIFM1 antibody (CAB2568) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] AIFM1 Polyclonal Antibody (CAB2568)

Immunohistochemistry of paraffin-embedded rat liver using AIFM1 antibody (CAB2568) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] AIFM1 Polyclonal Antibody (CAB2568)

Immunohistochemistry of paraffin-embedded human tonsil using AIFM1 antibody (CAB2568) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] AIFM1 Polyclonal Antibody (CAB2568)

Immunohistochemistry of paraffin-embedded human liver cancer using AIFM1 antibody (CAB2568) at dilution of 1:100 (40x lens).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Application:
IP
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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