The cookie settings on this website are set to 'allow all cookies' to give you the very best experience. Please click Accept Cookies to continue to use the site.

[KO Validated] ADRBK1 Polyclonal Antibody (CAB1662)

(No reviews yet) Write a Review
SKU:
CAB1662
  • Western blot - [KO Validated] ADRBK1 Polyclonal Antibody (CAB1662)
  • Western blot - [KO Validated] ADRBK1 Polyclonal Antibody (CAB1662)
  • Immunohistochemistry - [KO Validated] ADRBK1 Polyclonal Antibody (CAB1662)
  • Immunohistochemistry - [KO Validated] ADRBK1 Polyclonal Antibody (CAB1662)
  • Immunohistochemistry - [KO Validated] ADRBK1 Polyclonal Antibody (CAB1662)
  • Immunohistochemistry - [KO Validated] ADRBK1 Polyclonal Antibody (CAB1662)
$553.89

Description

Overview

Title:[KO Validated] ADRBK1 Polyclonal Antibody (CAB1662)
Size:100µL
Code:CAB1662
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 440-689 of human ADRBK1 (NP_001610.2).
GeneID:156
SwissProt:P25098
Synonyms:GRK2; ADRBK1; BARK1; BETA-ARK1; beta-adrenergic receptor kinase 1
Calculated MW:79kDa
Observed MW:80kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:HT-29, Mouse spleen, Mouse brain
Cellular Location:Cell membrane, Cytoplasm

Antibody Images

Western blot - [KO Validated] ADRBK1 Polyclonal Antibody (CAB1662)

Western blot analysis of extracts of various cell lines, using ADRBK1 antibody (CAB1662) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Western blot - [KO Validated] ADRBK1 Polyclonal Antibody (CAB1662)

Western blot analysis of extracts from normal (control) and ADRBK1 knockout (KO) 293T cells, using ADRBK1 antibody (CAB1662) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Immunohistochemistry - [KO Validated] ADRBK1 Polyclonal Antibody (CAB1662)

Immunohistochemistry of paraffin-embedded rat brain using ADRBK1 Antibody (CAB1662) at dilution of 1:200 (40x lens).

Immunohistochemistry - [KO Validated] ADRBK1 Polyclonal Antibody (CAB1662)

Immunohistochemistry of paraffin-embedded rat heart using ADRBK1 Antibody (CAB1662) at dilution of 1:200 (40x lens).

Immunohistochemistry - [KO Validated] ADRBK1 Polyclonal Antibody (CAB1662)

Immunohistochemistry of paraffin-embedded human esophageal cancer using ADRBK1 Antibody (CAB1662) at dilution of 1:200 (40x lens).

Immunohistochemistry - [KO Validated] ADRBK1 Polyclonal Antibody (CAB1662)

Immunohistochemistry of paraffin-embedded mouse kidney using ADRBK1 Antibody (CAB1662) at dilution of 1:200 (40x lens).

Datasheets

Antibody DatasheetAntibody MSDS

View AllClose

Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
View AllClose