Knockout Validated Antibodies

How do we validate our antibodies?

Using knockout cell lines (cells with no or reduced expression of precise target proteins) is the new gold standard to validate the specificity of antibodies.

We have created 100s of CRISPR Knockout cell lines by introducing frame-shift mutations into the genomic DNA of target cells using sgRNA/CAS9.

The CRISPR knockout cells were lysed and Western  Blotting was performed using Wild-Type (WT) cells as a control.

The Western Blots were then probed using our premium antibodies and visualisation of decreased protein expression was indicative of highly specific antibodies (Knockout Validated Antibodies).

How do I order?

You can order your knockout validated antibody and get your free antibody by emailing us at info@reagentgenie.com or by filling out our contact form below.

If you are purchasing online you can use discount code “KOVAL18” when checking out to avail of your free antibody when you purchase your antibody.

We have distributors throughout Europe and supply directly to North America and directly around the world.