How do we validate our antibodies?

Using knockout cell lines (cells with no or reduced expression of precise target proteins) is the new gold standard to validate the specificity of antibodies.

We have created 100s of CRISPR Knockout cell lines by introducing frame-shift mutations into the genomic DNA of target cells using sgRNA/CAS9.

The CRISPR knockout cells were lysed and Western  Blotting was performed using Wild-Type (WT) cells as a control.

The Western Blots were then probed using our premium antibodies and visualisation of decreased protein expression was indicative of highly specific antibodies (Knockout Validated Antibodies).

How do I order?

You can order your knockout validated antibody and get your free antibody by emailing us at info@reagentgenie.com or by filling out our contact form below.

If you are purchasing online you can use discount code “KOVAL18” when checking out to avail of your free antibody when you purchase your antibody.

We have distributors throughout Europe and supply directly to North America and directly around the world.

Knockout validated antibodies

p53 antibody (CDPAB05821) Knockout Varification:

Lane 1: Wild-type MCF7 cell lysate;
Lane 2: TP53 knockout MCF7 cell lysate;

Ab: 1µg/ml Rabbit Anti-Human TP53 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

Caspase 2 ( CDPAB08196) Knockout Varification:

Lane 1: Wild-type Jurkat cell lysate;
Lane 2: CASP2 knockout Jurkat cell lysate;

Ab: 1µg/ml Rabbit Anti-Human CASP2 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

Top Validated knockout antibodies – A to X

5′-Nucleotidase, Ecto (NT5E) polyclonal antibody Galectin 3 (GAL3) polyclonal antibody Phosphofructokinase, Platelet (PFKP) polyclonal antibody
Acid Phosphatase 1 (ACP1) polyclonal antibody GDP Dissociation Inhibitor 1 (GDI1) polyclonal antibody Phosphoglucomutase 1 (PGM1) polyclonal antibody
Acidic Nuclear Phosphoprotein 32 Family, Member A (ANP32A) polyclonal antibody General Transcription Factor IIIA (GTF3A) polyclonal antibody Phosphoglycerate Kinase 1 (PGK1) polyclonal antibody
Actinin Alpha 2 (ACTN2) polyclonal antibody Glucose 6 Phosphate Dehydrogenase (G6PD) polyclonal antibody Phosphoglycerate Mutase 1, Brain (PGAM1) polyclonal antibody
Actinin Alpha 4 (ACTN4) polyclonal antibody Glutaminase (GLS) polyclonal antibody Phosphoglycerate Mutase 2, Muscle (PGAM2) polyclonal antibody
Activated Leukocyte Cell Adhesion Molecule (ALCAM) polyclonal antibody Glutathione Peroxidase 4 (GPX4) polyclonal antibody Phosphohistidine Phosphatase 1 (PHPT1) polyclonal antibody
Adducin 1 (ADD1) polyclonal antibody Glutathione Reductase (GR) polyclonal antibody Phosphoserine Aminotransferase 1 (PSAT1) polyclonal antibody
Adhesion Regulating Molecule 1 (ADRM1) polyclonal antibody Glycogen Phosphorylase, Brain (PYGB) polyclonal antibody Platelet Membrane Glycoprotein IV (GP4) polyclonal antibody
Aldose Reductase Like Protein 1 (ARL1) polyclonal antibody Glycogen Phosphorylase, Liver (PYGL) polyclonal antibody Prenylcysteine Oxidase 1 (PCYOX1) polyclonal antibody
Alpha-Fetoprotein (aFP) polyclonal antibody Glycogen Phosphorylase, Liver (PYGL) polyclonal antibody Programmed Cell Death Protein 1 (PDCD1) polyclonal antibody
Alpha-Fetoprotein (aFP) polyclonal antibody Grancalcin (GCA) polyclonal antibody Programmed Cell Death Protein 1 Ligand 1 (PDCD1LG1) polyclonal antibody
Aminoacylase 1 (ACY1) polyclonal antibody Granulin (GRN) polyclonal antibody Programmed Cell Death Protein 6 (PDCD6) polyclonal antibody
Ancient Ubiquitous Protein 1 (AUP1) polyclonal antibody Granzyme A (GZMA) polyclonal antibody Prohibitin (PHB) polyclonal antibody
Angiopoietin 2 (ANGPT2) polyclonal antibody Granzyme M (GZMM) polyclonal antibody Prosaposin (PSAP) polyclonal antibody
Annexin A1 (ANXA1) monoclonal Antibody Growth Factor Receptor Bound Protein 10 (Grb10) polyclonal antibody Prostaglandin Endoperoxide Synthase 2 (PTGS2) polyclonal antibody
Annexin A3 (ANXA3) monoclonal Antibody Guanine Deaminase (GDA) polyclonal antibody Proteasome 26S Subunit, ATPase 1 (PSMC1) polyclonal antibody
Annexin A9 (ANXA9) polyclonal antibody Guanylate Binding Protein 4 (GBP4) polyclonal antibody Proteasome 26S Subunit, ATPase 2 (PSMC2) polyclonal antibody
Anthrax Toxin Receptor 2 (ANTXR2) polyclonal antibody Heat Shock 27kDa Protein 1 (HSPB1) polyclonal antibody Proteasome 26S Subunit, Non ATPase 5 (PSMD5) polyclonal antibody
Apoptosis Inducing Factor (AIF) polyclonal antibody Heat Shock 60kD Protein 1, Chaperonin (HSPD1) polyclonal antibody Proteasome 26S Subunit, Non ATPase 6 (PSMD6) polyclonal antibody
Aryl Hydrocarbon Receptor (AhR) polyclonal antibody Heat Shock 70kDa Protein 1 Like Protein (HSPA1L) monoclonal Antibody Proteasome 26S Subunit, Non ATPase 9 (PSMD9) polyclonal antibody
Asparagine Synthetase (ASNS) polyclonal antibody Heat Shock 70kDa Protein 1 Like Protein (HSPA1L) polyclonal antibody Proteasome Subunit Alpha Type 5 (PSMa5) polyclonal antibody
Asparaginyl tRNA Synthetase (NARS) polyclonal antibody Heat Shock 70kDa Protein 1A (HSPA1A) polyclonal antibody Proteasome Subunit Beta Type 6 (PSMb6) polyclonal antibody
Aspartyl tRNA Synthetase (DARS) polyclonal antibody Heat Shock Protein 40 (HSP40) polyclonal antibody Protein Disulfide Isomerase A4 (PDIA4) polyclonal antibody
ATP Binding Cassette Transporter B8 (ABCB8) polyclonal antibody Heparanase (HPA) polyclonal antibody Protein O-Fucosyltransferase 1 (POFUT1) polyclonal antibody
ATP Binding Cassette Transporter D2 (ABCD2) polyclonal antibody Heterogeneous Nuclear Ribonucleoprotein A2/B1 (HNRPA2B1) polyclonal antibody Protein Tyrosine Phosphatase F Interacting Protein 1 (PPFIA1) polyclonal antibody
ATP Binding Cassette Transporter F1 (ABCF1) polyclonal antibody Hexokinase 2 (HK2) polyclonal antibody Proteolipid Protein 2, Colonic Epithelium Enriched (PLP2) polyclonal antibody
ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3) polyclonal antibody High Mobility Group Box Protein 3 (HMGB3) polyclonal antibody Pyruvate Kinase, Muscle (PKM2) polyclonal antibody
Azurocidin 1 (AZU1) polyclonal antibody High Mobility Group Protein 1 (HMG1) polyclonal antibody RAD51 Homolog (RAD51) polyclonal antibody
B-Cell Leukemia/Lymphoma 2 (Bcl2) polyclonal antibody High Mobility Group Protein 1 (HMG1) polyclonal antibody RAR Related Orphan Receptor Alpha (RORa) polyclonal antibody
B-Cell Leukemia/Lymphoma 2 (Bcl2) polyclonal antibody High Mobility Group Protein 1 (HMG1) polyclonal antibody Receptor Activator Of Nuclear Factor Kappa B (RANk) polyclonal antibody
Beclin 1 (BECN1) polyclonal antibody High Mobility Group Protein 20B (HMG20B) polyclonal antibody Receptor I For The Fc Region Of Immunoglobulin G (FcgRI) polyclonal antibody
BH3 Interacting Domain Death Agonist (Bid) polyclonal antibody High Mobility Group Protein 20B (HMG20B) polyclonal antibody Receptor Interacting Serine Threonine Kinase 2 (RIPK2) polyclonal antibody
Biliverdin Reductase B (BLVRB) polyclonal antibody Histidine Decarboxylase (HDC) polyclonal antibody Recoverin (RCVRN) polyclonal antibody
Calcium Modulating Ligand (CAMLG) polyclonal antibody Histidine Rich Glycoprotein (HRG) polyclonal antibody Retinol Binding Protein 4, Plasma (RBP4) polyclonal antibody
Calmodulin Like Protein 3 (CALML3) polyclonal antibody Hyaluronan Binding Protein 1 (HABP1) polyclonal antibody Rh Blood Group, D Antigen (RHD) polyclonal antibody
Calmodulin Like Protein 5 (CALML5) polyclonal antibody Immunoglobulin Superfamily, Member 16 (IGSF16) polyclonal antibody Ribonuclease H2 Subunit A (RNASEH2A) polyclonal antibody
Calpain 9 (CAPN9) polyclonal antibody Inducible T-Cell Co Stimulator Ligand (ICOSLG) polyclonal antibody S100 Calcium Binding Protein A2 (S100A2) polyclonal antibody
Calumenin (CALU) polyclonal antibody Inhibitor Of Apoptosis Protein, X-Linked (XIAP) polyclonal antibody S100 Calcium Binding Protein A6 (S100A6) polyclonal antibody
Canopy 2 Homolog (CNPY2) polyclonal antibody Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A) polyclonal antibody Secernin 1 (SCRN1) polyclonal antibody
Carcinoembryonic Antigen (CEA) polyclonal antibody Insulin Degrading Enzyme (IDE) polyclonal antibody Secreted Frizzled Related Protein 4 (SFRP4) monoclonal Antibody
Caspase 1 (CASP1) polyclonal antibody Integrin Alpha D (ITGaD) polyclonal antibody Secreted Frizzled Related Protein 4 (SFRP4) polyclonal antibody
Caspase 1 (CASP1) polyclonal antibody Integrin Alpha V (ITGaV) polyclonal antibody Serine Hydroxymethyltransferase 2, Mitochondrial (SHMT2) polyclonal antibody
Caspase 2 (CASP2) polyclonal antibody Interferon Inducible Protein 35 (IFI35) polyclonal antibody Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1) polyclonal antibody
Caspase 3 (CASP3) polyclonal antibody Interferon Regulatory Factor 6 (IRF6) polyclonal antibody SHC-Transforming Protein 3 (SHC3) polyclonal antibody
Caspase 8 (CASP8) polyclonal antibody Interleukin 1 Receptor Type I (IL1R1) polyclonal antibody Sialic Acid Binding Ig Like Lectin 7 (SIGLEC7) polyclonal antibody
Caspase 8 (CASP8) polyclonal antibody Interleukin 31 Receptor A (IL31RA) polyclonal antibody Signal Recognition Particle Receptor B (SRPRB) polyclonal antibody
Caspase 9 (CASP9) polyclonal antibody Interleukin 7 Receptor (IL7R) polyclonal antibody Signal Regulatory Protein Alpha (SIRPa) polyclonal antibody
Caspase Recruitment Domain Family, Member 9 (CARD9) polyclonal antibody Isocitrate Dehydrogenase 1, Soluble (IDH1) polyclonal antibody Signal Transducer And Activator Of Transcription 4 (STAT4) polyclonal antibody
Catalase (CAT) polyclonal antibody Isocitrate Dehydrogenase 2, mitochondrial (IDH2) polyclonal antibody Signal Transducer And Activator Of Transcription 5A (STAT5A) polyclonal antibody
Cathepsin Z (CTSZ) polyclonal antibody Janus Kinase 2 (JAK2) polyclonal antibody Signal Transducer And Activator Of Transcription 5B (STAT5B) polyclonal antibody
Centromere Protein H (CENPH) polyclonal antibody Kallikrein 11 (KLK11) polyclonal antibody Small Nuclear Ribonucleoprotein Polypeptide D1 (SNRPD1) polyclonal antibody
Chaperonin Containing TCP1, Subunit 2 (CCT2) polyclonal antibody Karyopherin Alpha 2 (KPNa2) polyclonal antibody Solute Carrier Family 30 Member 8 (SLC30A8) polyclonal antibody
Chordin Like Protein 1 (CHRDL1) polyclonal antibody Kelch Like ECH Associated Protein 1 (KEAP1) polyclonal antibody Sp100 Nuclear Antigen (Sp100) polyclonal antibody
Chromobox Homolog 3 (CBX3) polyclonal antibody Keratin 1 (KRT1) polyclonal antibody S-Phase Kinase Associated Protein 1 (SKP1) polyclonal antibody
Cluster Of Differentiation 19 (CD19) polyclonal antibody Keratin 18 (KRT18) polyclonal antibody Spleen Tyrosine Kinase (SYK) polyclonal antibody
Cluster Of Differentiation 276 (CD276) polyclonal antibody Keratin 18 (KRT18) polyclonal antibody Sprouty Homolog 2 (SPRY2) polyclonal antibody
Coactosin Like Protein 1 (COTL1) polyclonal antibody Keratin 19 (KRT19) polyclonal antibody Stratifin (SFN) polyclonal antibody
Collagen Type III Alpha 1 (COL3a1) polyclonal antibody Keratin 2 (KRT2) polyclonal antibody Stress Induced Phosphoprotein 1 (STIP1) polyclonal antibody
Complement Component 2 (C2) polyclonal antibody Keratin 8 (KRT8) polyclonal antibody Stromal Cell Derived Factor 2 Like Protein 1 (SDF2L1) polyclonal antibody
Corticosteroid Binding Globulin (CBG) polyclonal antibody Lactate Dehydrogenase B (LDHB) polyclonal antibody Structural Maintenance Of Chromosomes Protein 3 (SMC3) polyclonal antibody
Cyclin B (CCNB) polyclonal antibody Laminin Gamma 2 (LAMC2) polyclonal antibody Superoxide Dismutase 2, Mitochondrial (SOD2) polyclonal antibody
Cyclin D1 (CCND1) polyclonal antibody Lectin, Mannose Binding 1 (LMAN1) polyclonal antibody Superoxide Dismutase 2, Mitochondrial (SOD2) polyclonal antibody
Cyclin Dependent Kinase 18 (CDK18) polyclonal antibody Lymphotoxin Beta Receptor (LTbR) polyclonal antibody Suprabasin (SBSN) polyclonal antibody
Cyclin Dependent Kinase 4 (CDK4) polyclonal antibody Lysine Specific Demethylase 4A (KDM4A) polyclonal antibody Survival Of Motor Neuron 1, Telomeric (SMN1) polyclonal antibody
Cyclophilin D (CYPD) polyclonal antibody Lysyl tRNA Synthetase (KARS) polyclonal antibody Survival Of Motor Neuron 2, Centromeric (SMN2) polyclonal antibody
Cyclophilin D (CYPD) polyclonal antibody Macrophage Migration Inhibitory Factor (MIF) polyclonal antibody Syntaxin 2 (STX2) polyclonal antibody
Cystatin B (CSTB) polyclonal antibody Major Histocompatibility Complex Class I C (MHCC) polyclonal antibody Syntenin 1 (ST1) polyclonal antibody
Cysteine And Glycine Rich Protein 1 (CSRP1) polyclonal antibody Major Histocompatibility Complex Class II DR Alpha (MHCDRa) polyclonal antibody Tachykinin Receptor 2 (TACR2) polyclonal antibody
Cytochrome P450 26A1 (CYP26A1) polyclonal antibody Mannose Associated Serine Protease 1 (MASP1) polyclonal antibody Target Of The Antiproliferative Antibody 1 (TAPA1) polyclonal antibody
Cytokeratin Fragment Antigen 21-1 (CYFRA21-1) polyclonal antibody Mannose Phosphate Isomerase (MPI) polyclonal antibody Taxilin Alpha (TXLNa) polyclonal antibody
Cytoplasmic Antiproteinase 3 (CAP3) polyclonal antibody Matrix Metalloproteinase 9 (MMP9) monoclonal Antibody T-Box Protein 3 (TBX3) polyclonal antibody
Deoxyribonuclease I (DNASE1) polyclonal antibody Matrix Metalloproteinase 9 (MMP9) polyclonal antibody Thiosulfate Sulfurtransferase (TST) polyclonal antibody
Dihydrolipoyl Dehydrogenase (DLD) polyclonal antibody Matrix Metalloproteinase 9 (MMP9) polyclonal antibody Thymidine Kinase 1, Soluble (TK1) monoclonal Antibody
Dipeptidyl Peptidase 3 (DPP3) polyclonal antibody Membrane Protein, Palmitoylated 6 (MPP6) polyclonal antibody Thymidine Kinase 1, Soluble (TK1) polyclonal antibody
Discs, Large Homolog 5 (DLG5) polyclonal antibody Methionyl tRNA Synthetase (MARS) polyclonal antibody Thymidylate Synthetase (TYMS) polyclonal antibody
Dopamine Beta Hydroxylase (DbH) polyclonal antibody Methionyl tRNA Synthetase (MARS) polyclonal antibody Thyroxine Binding Globulin (TBG) polyclonal antibody
Dyskerin (DKC) polyclonal antibody Minichromosome Maintenance Deficient 2 (MCM2) polyclonal antibody TNF Receptor Associated Factor 4 (TRAF4) polyclonal antibody
Early B-Cell Factor 1 (EBF1) polyclonal antibody Mitochondrial Ribosomal Protein L1 (MRPL1) polyclonal antibody TNF Receptor Associated Factor 6 (TRAF6) polyclonal antibody
Early Growth Response Protein 2 (EGR2) polyclonal antibody Mitogen Activated Protein Kinase Kinase 2 (MAP2K2) polyclonal antibody Toll Like Receptor 5 (TLR5) polyclonal antibody
Enolase, Neuron Specific (NSE) polyclonal antibody Mothers Against Decapentaplegic Homolog 1 (Smad1) polyclonal antibody Transglutaminase 2, Tissue (TGM2) polyclonal antibody
Enolase, Neuron Specific (NSE) polyclonal antibody Mothers Against Decapentaplegic Homolog 3 (Smad3) polyclonal antibody Transketolase (TKT) polyclonal antibody
Enolase, Neuron Specific (NSE) polyclonal antibody Myxovirus Resistance 1 (MX1) polyclonal antibody Transketolase (TKT) polyclonal antibody
Enolase, Non Neuronal (NNE) polyclonal antibody NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1) polyclonal antibody TTK Protein Kinase (TTK) polyclonal antibody
Ephrin Type A Receptor 10 (EPHA10) polyclonal antibody Neuro Oncological Ventral Antigen 1 (NOVA1) polyclonal antibody Tumor Necrosis Factor Receptor Superfamily, Member 10B (TNFRSF10B) polyclonal antibody
Epithelial Stromal Interaction 1, Breast (EPSTI1) polyclonal antibody Nicotinamide-N-Methyltransferase (NNMT) polyclonal antibody Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) polyclonal antibody
Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1) polyclonal antibody Nitric Oxide Synthase Interacting Protein (NOSIP) polyclonal antibody Tumor Protein p53 (TP53) polyclonal antibody
Exportin 1 (XPO1) polyclonal antibody NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) polyclonal antibody Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) polyclonal antibody
Fas Associating Death Domain Containing Protein (FADD) polyclonal antibody Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1) polyclonal antibody Ubiquitin Conjugating Enzyme E2I (UBE2I) polyclonal antibody
Fibrillarin (FBL) polyclonal antibody Nucleophosmin (NPM) polyclonal antibody Ubiquitin Conjugating Enzyme E2L3 (UBE2L3) polyclonal antibody
Fibrinogen Beta (FGb) polyclonal antibody Numb Homolog (NUMB) polyclonal antibody Ubiquitin Conjugating Enzyme E2S (UBE2S) polyclonal antibody
FK506 Binding Protein 5 (FKBP5) polyclonal antibody O-6-Methylguanine DNA Methyltransferase (MGMT) polyclonal antibody UDP-Glucose Glycoprotein Glucosyltransferase 1 (UGGT1) polyclonal antibody
FK506 Binding Protein 7 (FKBP7) polyclonal antibody Oncoprotein Induced Transcript 3 (OIT3) polyclonal antibody Valosin Containing Protein (VCP) polyclonal antibody
FK506 Binding Protein Like Protein (FKBPL) polyclonal antibody p21 Protein Activated Kinase 1 (PAK1) polyclonal antibody Vanin 1 (VNN1) polyclonal antibody
Focal Adhesion Kinase (FAK) polyclonal antibody p21 Protein Activated Kinase 2 (PAK2) polyclonal antibody Vascular Endothelial Growth Factor C (VEGFC) polyclonal antibody
Folate Receptor 1, Adult (FOLR1) polyclonal antibody p21 Protein Activated Kinase 4 (PAK4) polyclonal antibody Visfatin (VF) polyclonal antibody
Fumarase (FUM) polyclonal antibody Palladin (PALLD) polyclonal antibody V-Myb Myeloblastosis Viral Oncogene Homolog (MYB) polyclonal antibody
Fumarase (FUM) polyclonal antibody Parkinson Disease Protein 7 (PARK7) polyclonal antibody Wiskott Aldrich Syndrome Protein (WASP) polyclonal antibody
Galectin 1 (GAL1) polyclonal antibody Peroxisomal Biogenesis Factor 2 (PEX2) polyclonal antibody Xylosyltransferase II (XYLT2) polyclonal antibody
Phenylalanine Hydroxylase (PAH) polyclonal antibody
Phosphatidylserine Receptor (PSR) polyclonal antibody

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*Terms & conditions

Terms & Conditions of Offer: A purchase of an Antibody Genie knockout validated antibody is required for customers to avail of a free antibody |Only 1 free 10ug antibody pack per lab | Does not include shipping costs | Reagent Genie reserves the right to change this offer at any time | Valid until 30/04/2018 | Free trial antibodies only available to first 200 customers | Prices correct as of 01/01/2018 | Antibody Genie is a registered Trade mark of Reagent Genie Limited