How do we validate our antibodies?
Using knockout cell lines (cells with no or reduced expression of precise target proteins) is the new gold standard to validate the specificity of antibodies.
We have created 100s of CRISPR Knockout cell lines by introducing frame-shift mutations into the genomic DNA of target cells using sgRNA/CAS9.
The CRISPR knockout cells were lysed and Western Blotting was performed using Wild-Type (WT) cells as a control.
The Western Blots were then probed using our premium antibodies and visualisation of decreased protein expression was indicative of highly specific antibodies (Knockout Validated Antibodies).
How do I order?
You can order your knockout validated antibody and get your free antibody by emailing us at firstname.lastname@example.org or by filling out our contact form below.
If you are purchasing online you can use discount code “KOVAL18” when checking out to avail of your free antibody when you purchase your antibody.
We have distributors throughout Europe and supply directly to North America and directly around the world.
Knockout validated antibodies
p53 antibody (CDPAB05821) Knockout Varification:
Lane 1: Wild-type MCF7 cell lysate;
Lane 2: TP53 knockout MCF7 cell lysate;
Ab: 1µg/ml Rabbit Anti-Human TP53 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Caspase 2 ( CDPAB08196) Knockout Varification:
Lane 1: Wild-type Jurkat cell lysate;
Lane 2: CASP2 knockout Jurkat cell lysate;
Ab: 1µg/ml Rabbit Anti-Human CASP2 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Top Validated knockout antibodies – A to X
Contact us for a quote!
*Terms & conditions
Terms & Conditions of Offer: A purchase of an Antibody Genie knockout validated antibody is required for customers to avail of a free antibody |Only 1 free 10ug antibody pack per lab | Does not include shipping costs | Reagent Genie reserves the right to change this offer at any time | Valid until 30/04/2018 | Free trial antibodies only available to first 200 customers | Prices correct as of 01/01/2018 | Antibody Genie is a registered Trade mark of Reagent Genie Limited