How do we validate our antibodies?

Using knockout cell lines (cells with no or reduced expression of precise target proteins) is the new gold standard to validate the specificity of antibodies.

We have created 100s of CRISPR Knockout cell lines by introducing frame-shift mutations into the genomic DNA of target cells using sgRNA/CAS9.

The CRISPR knockout cells were lysed and Western  Blotting was performed using Wild-Type (WT) cells as a control.

The Western Blots were then probed using our premium antibodies and visualisation of decreased protein expression was indicative of highly specific antibodies (Knockout Validated Antibodies).

How do I order?

You can order your knockout validated antibody and get your free antibody by emailing us at or by filling out our contact form below.

If you are purchasing online you can use discount code “KOVAL18” when checking out to avail of your free antibody when you purchase your antibody.

We have distributors throughout Europe and supply directly to North America and directly around the world.

Knockout validated antibodies

p53 antibody (CDPAB05821) Knockout Varification:

Lane 1: Wild-type MCF7 cell lysate;
Lane 2: TP53 knockout MCF7 cell lysate;

Ab: 1µg/ml Rabbit Anti-Human TP53 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

Caspase 2 ( CDPAB08196) Knockout Varification:

Lane 1: Wild-type Jurkat cell lysate;
Lane 2: CASP2 knockout Jurkat cell lysate;

Ab: 1µg/ml Rabbit Anti-Human CASP2 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

Top Validated knockout antibodies – A to X

A – GG – PP – X
5′-Nucleotidase, Ecto (NT5E)GDP Dissociation Inhibitor 1 (GDI1)Phosphofructokinase, Platelet (PFKP)
Acid Phosphatase 1 (ACP1)General Transcription Factor IIIA (GTF3A)Phosphoglucomutase 1 (PGM1)
Acidic Nuclear Phosphoprotein 32 Family, Member A (ANP32A)Glucose 6 Phosphate Dehydrogenase (G6PD)Phosphoglycerate Kinase 1 (PGK1)
Actinin Alpha 2 (ACTN2)Glutaminase (GLS)Phosphoglycerate Mutase 1, Brain (PGAM1)
Actinin Alpha 4 (ACTN4)Glutathione Peroxidase 4 (GPX4)Phosphoglycerate Mutase 2, Muscle (PGAM2)
Activated Leukocyte Cell Adhesion Molecule (ALCAM)Glutathione Reductase (GR)Phosphohistidine Phosphatase 1 (PHPT1)
Adducin 1 (ADD1)Glycogen Phosphorylase, Brain (PYGB)Phosphoserine Aminotransferase 1 (PSAT1)
Adhesion Regulating Molecule 1 (ADRM1)Glycogen Phosphorylase, Liver (PYGL)Platelet Membrane Glycoprotein IV (GP4)
Aldose Reductase Like Protein 1 (ARL1)Glycogen Phosphorylase, Liver (PYGL)Prenylcysteine Oxidase 1 (PCYOX1)
Alpha-Fetoprotein (aFP)Grancalcin (GCA)Programmed Cell Death Protein 1 (PDCD1)
Alpha-Fetoprotein (aFP)Granulin (GRN)Programmed Cell Death Protein 1 Ligand 1 (PDCD1LG1)
Aminoacylase 1 (ACY1)Granzyme A (GZMA)Programmed Cell Death Protein 6 (PDCD6)
Ancient Ubiquitous Protein 1 (AUP1)Granzyme M (GZMM)Prohibitin (PHB)
Angiopoietin 2 (ANGPT2)Growth Factor Receptor Bound Protein 10 (Grb10)Prosaposin (PSAP)
Annexin A1 (ANXA1)Guanine Deaminase (GDA)Prostaglandin Endoperoxide Synthase 2 (PTGS2)
Annexin A3 (ANXA3)Guanylate Binding Protein 4 (GBP4)Proteasome 26S Subunit, ATPase 1 (PSMC1)
Annexin A9 (ANXA9)Heat Shock 27kDa Protein 1 (HSPB1)Proteasome 26S Subunit, ATPase 2 (PSMC2)
Anthrax Toxin Receptor 2 (ANTXR2)Heat Shock 60kD Protein 1, Chaperonin (HSPD1)Proteasome 26S Subunit, Non ATPase 5 (PSMD5)
Anti-Caspase 1 (CASP1)Heat Shock 70kDa Protein 1 Like Protein (HSPA1L)Proteasome 26S Subunit, Non ATPase 6 (PSMD6)
Apoptosis Inducing Factor (AIF)Heat Shock 70kDa Protein 1 Like Protein (HSPA1L)Proteasome 26S Subunit, Non ATPase 9 (PSMD9)
Aryl Hydrocarbon Receptor (AhR)Heat Shock 70kDa Protein 1A (HSPA1A)Proteasome Subunit Alpha Type 5 (PSMa5)
Asparagine Synthetase (ASNS)Heat Shock Protein 40 (HSP40)Proteasome Subunit Beta Type 6 (PSMb6)
Asparaginyl tRNA Synthetase (NARS)Heparanase (HPA)Protein Disulfide Isomerase A4 (PDIA4)
Aspartyl tRNA Synthetase (DARS)Heterogeneous Nuclear Ribonucleoprotein A2/B1 (HNRPA2B1)Protein O-Fucosyltransferase 1 (POFUT1)
ATP Binding Cassette Transporter B8 (ABCB8)Hexokinase 2 (HK2)Protein Tyrosine Phosphatase F Interacting Protein 1 (PPFIA1)
ATP Binding Cassette Transporter D2 (ABCD2)High Mobility Group Box Protein 3 (HMGB3)Proteolipid Protein 2, Colonic Epithelium Enriched (PLP2)
ATP Binding Cassette Transporter F1 (ABCF1)High Mobility Group Protein 1 (HMG1)Pyruvate Kinase, Muscle (PKM2)
ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3)High Mobility Group Protein 1 (HMG1)RAD51 Homolog (RAD51)
Azurocidin 1 (AZU1)High Mobility Group Protein 1 (HMG1)RAR Related Orphan Receptor Alpha (RORa)
B-Cell Leukemia/Lymphoma 2 (Bcl2)High Mobility Group Protein 20B (HMG20B)Receptor Activator Of Nuclear Factor Kappa B (RANk)
B-Cell Leukemia/Lymphoma 2 (Bcl2)High Mobility Group Protein 20B (HMG20B)Receptor I For The Fc Region Of Immunoglobulin G (FcgRI)
Beclin 1 (BECN1)Histidine Decarboxylase (HDC)Receptor Interacting Serine Threonine Kinase 2 (RIPK2)
BH3 Interacting Domain Death Agonist (Bid)Histidine Rich Glycoprotein (HRG)Recoverin (RCVRN)
Biliverdin Reductase B (BLVRB)Hyaluronan Binding Protein 1 (HABP1)Retinol Binding Protein 4, Plasma (RBP4)
Calcium Modulating Ligand (CAMLG)Immunoglobulin Superfamily, Member 16 (IGSF16)Rh Blood Group, D Antigen (RHD)
Calmodulin Like Protein 3 (CALML3)Inducible T-Cell Co Stimulator Ligand (ICOSLG)Ribonuclease H2 Subunit A (RNASEH2A)
Calmodulin Like Protein 5 (CALML5)Inhibitor Of Apoptosis Protein, X-Linked (XIAP)S100 Calcium Binding Protein A2 (S100A2)
Calpain 9 (CAPN9)Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A)S100 Calcium Binding Protein A6 (S100A6)
Calumenin (CALU)Insulin Degrading Enzyme (IDE)Secernin 1 (SCRN1)
Canopy 2 Homolog (CNPY2)Integrin Alpha D (ITGaD)Secreted Frizzled Related Protein 4 (SFRP4)
Carcinoembryonic Antigen (CEA)Integrin Alpha V (ITGaV)Secreted Frizzled Related Protein 4 (SFRP4)
Caspase 1 (CASP1)Interferon Inducible Protein 35 (IFI35)Serine Hydroxymethyltransferase 2, Mitochondrial (SHMT2)
Caspase 2 (CASP2)Interferon Regulatory Factor 6 (IRF6)Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1)
Caspase 3 (CASP3)Interleukin 1 Receptor Type I (IL1R1)SHC-Transforming Protein 3 (SHC3)
Caspase 8 (CASP8)Interleukin 31 Receptor A (IL31RA)Sialic Acid Binding Ig Like Lectin 7 (SIGLEC7)
Caspase 8 (CASP8)Interleukin 7 Receptor (IL7R)Signal Recognition Particle Receptor B (SRPRB)
Caspase 9 (CASP9)Isocitrate Dehydrogenase 1, Soluble (IDH1)Signal Regulatory Protein Alpha (SIRPa)
Caspase Recruitment Domain Family, Member 9 (CARD9)Isocitrate Dehydrogenase 2, mitochondrial (IDH2)Signal Transducer And Activator Of Transcription 4 (STAT4)
Catalase (CAT)Janus Kinase 2 (JAK2)Signal Transducer And Activator Of Transcription 5A (STAT5A)
Cathepsin Z (CTSZ)Kallikrein 11 (KLK11)Signal Transducer And Activator Of Transcription 5B (STAT5B)
Centromere Protein H (CENPH)Karyopherin Alpha 2 (KPNa2)Small Nuclear Ribonucleoprotein Polypeptide D1 (SNRPD1)
Chaperonin Containing TCP1, Subunit 2 (CCT2)Kelch Like ECH Associated Protein 1 (KEAP1)Solute Carrier Family 30 Member 8 (SLC30A8)
Chordin Like Protein 1 (CHRDL1)Keratin 1 (KRT1)Sp100 Nuclear Antigen (Sp100)
Chromobox Homolog 3 (CBX3)Keratin 18 (KRT18)S-Phase Kinase Associated Protein 1 (SKP1)
Cluster Of Differentiation 19 (CD19)Keratin 18 (KRT18)Spleen Tyrosine Kinase (SYK)
Cluster Of Differentiation 276 (CD276)Keratin 19 (KRT19)Sprouty Homolog 2 (SPRY2)
Coactosin Like Protein 1 (COTL1)Keratin 2 (KRT2)Stratifin (SFN)
Collagen Type III Alpha 1 (COL3a1)Keratin 8 (KRT8)Stress Induced Phosphoprotein 1 (STIP1)
Complement Component 2 (C2)Lactate Dehydrogenase B (LDHB)Stromal Cell Derived Factor 2 Like Protein 1 (SDF2L1)
Corticosteroid Binding Globulin (CBG)Laminin Gamma 2 (LAMC2)Structural Maintenance Of Chromosomes Protein 3 (SMC3)
Cyclin B (CCNB)Lectin, Mannose Binding 1 (LMAN1)Superoxide Dismutase 2, Mitochondrial (SOD2)
Cyclin D1 (CCND1)Lymphotoxin Beta Receptor (LTbR)Superoxide Dismutase 2, Mitochondrial (SOD2)
Cyclin Dependent Kinase 18 (CDK18)Lysine Specific Demethylase 4A (KDM4A)Suprabasin (SBSN)
Cyclin Dependent Kinase 4 (CDK4)Lysyl tRNA Synthetase (KARS)Survival Of Motor Neuron 1, Telomeric (SMN1)
Cyclophilin D (CYPD)Macrophage Migration Inhibitory Factor (MIF)Survival Of Motor Neuron 2, Centromeric (SMN2)
Cyclophilin D (CYPD)Major Histocompatibility Complex Class I C (MHCC)Syntaxin 2 (STX2)
Cystatin B (CSTB)Major Histocompatibility Complex Class II DR Alpha (MHCDRa)Syntenin 1 (ST1)
Cysteine And Glycine Rich Protein 1 (CSRP1)Mannose Associated Serine Protease 1 (MASP1)Tachykinin Receptor 2 (TACR2)
Cytochrome P450 26A1 (CYP26A1)Mannose Phosphate Isomerase (MPI)Target Of The Antiproliferative Antibody 1 (TAPA1)
Cytokeratin Fragment Antigen 21-1 (CYFRA21-1)Matrix Metalloproteinase 9 (MMP9)Taxilin Alpha (TXLNa)
Cytoplasmic Antiproteinase 3 (CAP3)Matrix Metalloproteinase 9 (MMP9)T-Box Protein 3 (TBX3)
Deoxyribonuclease I (DNASE1)Matrix Metalloproteinase 9 (MMP9)Thiosulfate Sulfurtransferase (TST)
Dihydrolipoyl Dehydrogenase (DLD)Membrane Protein, Palmitoylated 6 (MPP6)Thymidine Kinase 1, Soluble (TK1)
Dipeptidyl Peptidase 3 (DPP3)Methionyl tRNA Synthetase (MARS)Thymidine Kinase 1, Soluble (TK1)
Discs, Large Homolog 5 (DLG5)Methionyl tRNA Synthetase (MARS)Thymidylate Synthetase (TYMS)
Dopamine Beta Hydroxylase (DbH)Minichromosome Maintenance Deficient 2 (MCM2)Thyroxine Binding Globulin (TBG)
Dyskerin (DKC)Mitochondrial Ribosomal Protein L1 (MRPL1)TNF Receptor Associated Factor 4 (TRAF4)
Early B-Cell Factor 1 (EBF1)Mitogen Activated Protein Kinase Kinase 2 (MAP2K2)TNF Receptor Associated Factor 6 (TRAF6)
Early Growth Response Protein 2 (EGR2)Mothers Against Decapentaplegic Homolog 1 (Smad1)Toll Like Receptor 5 (TLR5)
Enolase, Neuron Specific (NSE)Mothers Against Decapentaplegic Homolog 3 (Smad3)Transglutaminase 2, Tissue (TGM2)
Enolase, Neuron Specific (NSE)Myxovirus Resistance 1 (MX1)Transketolase (TKT)
Enolase, Neuron Specific (NSE)NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1)Transketolase (TKT)
Enolase, Non Neuronal (NNE)Neuro Oncological Ventral Antigen 1 (NOVA1)TTK Protein Kinase (TTK)
Ephrin Type A Receptor 10 (EPHA10)Nicotinamide-N-Methyltransferase (NNMT)Tumor Necrosis Factor Receptor Superfamily, Member 10B (TNFRSF10B)
Epithelial Stromal Interaction 1, Breast (EPSTI1)Nitric Oxide Synthase Interacting Protein (NOSIP)Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A)
Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1)NLR Family, Pyrin Domain Containing Protein 3 (NLRP3)Tumor Protein p53 (TP53)
Exportin 1 (XPO1)Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1)Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1)
Fas Associating Death Domain Containing Protein (FADD)Nucleophosmin (NPM)Ubiquitin Conjugating Enzyme E2I (UBE2I)
Fibrillarin (FBL)Numb Homolog (NUMB)Ubiquitin Conjugating Enzyme E2L3 (UBE2L3)
Fibrinogen Beta (FGb)O-6-Methylguanine DNA Methyltransferase (MGMT)Ubiquitin Conjugating Enzyme E2S (UBE2S)
FK506 Binding Protein 5 (FKBP5)Oncoprotein Induced Transcript 3 (OIT3)UDP-Glucose Glycoprotein Glucosyltransferase 1 (UGGT1)
FK506 Binding Protein 7 (FKBP7)p21 Protein Activated Kinase 1 (PAK1)Valosin Containing Protein (VCP)
FK506 Binding Protein Like Protein (FKBPL)p21 Protein Activated Kinase 2 (PAK2)Vanin 1 (VNN1)
Focal Adhesion Kinase (FAK)p21 Protein Activated Kinase 4 (PAK4)Vascular Endothelial Growth Factor C (VEGFC)
Folate Receptor 1, Adult (FOLR1)Palladin (PALLD)Visfatin (VF)
Fumarase (FUM)Parkinson Disease Protein 7 (PARK7)V-Myb Myeloblastosis Viral Oncogene Homolog (MYB)
Fumarase (FUM)Peroxisomal Biogenesis Factor 2 (PEX2)Wiskott Aldrich Syndrome Protein (WASP)
Galectin 1 (GAL1)Phenylalanine Hydroxylase (PAH)Xylosyltransferase II (XYLT2)
Galectin 3 (GAL3)Phosphatidylserine Receptor (PSR)

Contact us for a quote!

*Terms & conditions

Terms & Conditions of Offer: A purchase of an Antibody Genie knockout validated antibody is required for customers to avail of a free antibody |Only 1 free 10ug antibody pack per lab | Does not include shipping costs | Reagent Genie reserves the right to change this offer at any time | Valid until 30/04/2018 | Free trial antibodies only available to first 200 customers | Prices correct as of 01/01/2018 | Antibody Genie is a registered Trade mark of Reagent Genie Limited