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KLF2 Polyclonal Antibody (CAB16480)

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CAB16480
  • Western blot - KLF2 Polyclonal Antibody (CAB16480)
  • Western blot - KLF2 Polyclonal Antibody (CAB16480)
  • Immunofluorescence - KLF2 Polyclonal Antibody (CAB16480)
  • Immunofluorescence - KLF2 Polyclonal Antibody (CAB16480)
  • Immunofluorescence - KLF2 Polyclonal Antibody (CAB16480)
$331.89

Description

Overview

Title:KLF2 Polyclonal Antibody (CAB16480)
Size:100µL
Code:CAB16480
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a protein that belongs to the Kruppel family of transcription factors. The encoded zinc finger protein is expressed early in mammalian development and is found in many different cell types. The protein acts to bind the CACCC box found in the promoter of target genes to activate their transcription. It plays a role in many processes during development and disease including adipogenesis, embryonic erythropoiesis, epithelial integrity, inflammation and t-cell viability.

Immunogen Information

Immunogen:A synthetic peptide corresponding to a sequence within amino acids 50-150 of human KLF2 (NP_057354.1).
GeneID:10365
SwissProt:Q9Y5W3
Synonyms:KLF2; LKLF; Kruppel like factor 2
Calculated MW:37kDa
Observed MW:37kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IF
Recommended Dilution:WB 1:500 - 1:2000; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    
Positive Samples:PC-3, rat lung
Cellular Location:Nucleus

Antibody Images

Western blot - KLF2 Polyclonal Antibody (CAB16480)

Western blot analysis of extracts of various cell lines, using KLF2 antibody (CAB16480) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90S.

Western blot - KLF2 Polyclonal Antibody (CAB16480)

Western blot analysis of extracts of various cell lines, using KLF2 antibody (CAB16480) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.

Immunofluorescence - KLF2 Polyclonal Antibody (CAB16480)

Immunofluorescence analysis of C6 cells using KLF2 antibody (CAB16480) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence - KLF2 Polyclonal Antibody (CAB16480)

Immunofluorescence analysis of NIH/3T3 cells using KLF2 antibody (CAB16480) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence - KLF2 Polyclonal Antibody (CAB16480)

Immunofluorescence analysis of U2OS cells using KLF2 antibody (CAB16480) at dilution of 1:100. Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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