KEAP1 Polyclonal Antibody (CAB17061)
- SKU:
- CAB17061
Description
| Title: | KEAP1 Polyclonal Antibody (CAB17061) | |
| Size: | 100µL | |
| Code: | CAB17061 | |
| Host Species: | Rabbit | |
| Purification: | Affinity purification | |
| Isotype: | IgG |
Background
This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.
Immunogen Information
| Immunogen: | A synthetic peptide of human KEAP1. |
| GeneID: | 9817 |
| SwissProt: | Q14145 |
| Synonyms: | KEAP1; INrf2; KLHL19; kelch-like ECH-associated protein 1 |
| Calculated MW: | 69kDa |
| Observed MW: | Refer to figures |
Applications
| Reactivity: | Mouse |
| Tested Applications: | WB; IF; IP |
| Recommended Dilution: | WB 1:500 - 1:2000; IF 1:50 - 1:100; IP 1:50 - 1:100 |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Application Key: | Western blotting Immunofluorescence Immunoprecipitation |
| Positive Samples: | mouse brain |
| Cellular Location: | N/A |
Antibody Images
| Western blot analysis of extracts of mouse brain, using KEAP1 antibody (CAB17061) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5min. |
Datasheets
Additional Information
Antibody Type: |
Polyclonal |
Application: |
WB |
Application: |
IF |
Application: |
IP |
Reactivity: |
Mouse |
Host Species: |
Rabbit |
Isotype: |
IgG |
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