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HRP-conjugated Beta-Tubulin Monoclonal Antibody (CABC030)

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SKU:
CABC030
$331.89

Description

Overview

Title:HRP-conjugated Beta-Tubulin Monoclonal Antibody (CABC030)
Size:100uL
Code:CABC030
Host Species:Mouse
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13.

Immunogen Information

Immunogen:Recombinant protein of human TUBB
GeneID:203068
SwissProt:P07437
Synonyms:TUBB; CDCBM6; CSCSC1; M40; OK/SW-cl.56; TUBB1; TUBB5; tubulin beta chain
Calculated MW:50kDa
Observed MW:55kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB;
Recommended Dilution:WB 1:5000 - 1:20000
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles.Buffer: PBS, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:HeLa, U-87MG, 293T, Mouse liver, Mouse brain, Mouse kidney, Rat liver, Rat brain
Cellular Location:Cytoplasm, cytoskeleton

Antibody Images

Western blot - HRP-conjugated Beta-Tubulin Monoclonal Antibody (CABC030)

Western blot analysis of extracts of various cell lines, using HRP-Conjugated-Beta-Tubulin antibody (CABC030) at 1:10000 dilution.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
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Additional Information

ANTIBODY TYPE:
Monoclonal
APPLICATION:
WB
REACTIVITY:
Human
REACTIVITY:
Mouse
REACTIVITY:
Rat
HOST SPECIES:
Mouse
ISOTYPE:
IgG
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