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HDAC6 Polyclonal Antibody (CAB11259)

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CAB11259
  • Western blot - HDAC6 Polyclonal Antibody (CAB11259)
  • Immunofluorescence - HDAC6 Polyclonal Antibody (CAB11259)
  • Immunoprecipitation - HDAC6 Polyclonal Antibody (CAB11259)
$331.89

Description

Overview

Title:HDAC6 Polyclonal Antibody (CAB11259)
Size:100µL
Code:CAB11259
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 836-1104 of human HDAC6 (NP_006035.2).
GeneID:10013
SwissProt:Q9UBN7
Synonyms:HDAC6; CPBHM; HD6; JM21; PPP1R90; histone deacetylase 6
Calculated MW:114kDa/131kDa
Observed MW:170kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF; IP
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200; IP 1:20 - 1:50
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive Samples:U-87MG, SKOV3, HepG2, SW620, BT-474, Mouse kidney
Cellular Location:Cell projection, Cytoplasm, Nucleus, Perikaryon, axon, dendrite

Antibody Images

Western blot - HDAC6 Polyclonal Antibody (CAB11259)

Western blot analysis of extracts of various cell lines, using HDAC6 antibody (CAB11259) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Immunofluorescence - HDAC6 Polyclonal Antibody (CAB11259)

Immunofluorescence analysis of HeLa cells using HDAC6 antibody (CAB11259) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation - HDAC6 Polyclonal Antibody (CAB11259)

Immunoprecipitation analysis of 100ug extracts of HepG2 cells using 3ug HDAC6 antibody (CAB11259). Western blot was performed from the immunoprecipitate using HDAC6 antibody (CAB11259) at a dilition of 1:1000.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Application:
IP
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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