HDAC6 Polyclonal Antibody (CAB11259)
- SKU:
- CAB11259
Description
| Title: | HDAC6 Polyclonal Antibody (CAB11259) | |
| Size: | 100µL | |
| Code: | CAB11259 | |
| Host Species: | Rabbit | |
| Purification: | Affinity purification | |
| Isotype: | IgG |
Background
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription.
Immunogen Information
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 836-1104 of human HDAC6 (NP_006035.2). |
| GeneID: | 10013 |
| SwissProt: | Q9UBN7 |
| Synonyms: | HDAC6; CPBHM; HD6; JM21; PPP1R90; histone deacetylase 6 |
| Calculated MW: | 114kDa/131kDa |
| Observed MW: | 170kDa |
Applications
| Reactivity: | Human, Mouse, Rat |
| Tested Applications: | WB; IHC; IF; IP |
| Recommended Dilution: | WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200; IP 1:20 - 1:50 |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Application Key: | Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation |
| Positive Samples: | U-87MG, SKOV3, HepG2, SW620, BT-474, Mouse kidney |
| Cellular Location: | Cell projection, Cytoplasm, Nucleus, Perikaryon, axon, dendrite |
Antibody Images
| Western blot analysis of extracts of various cell lines, using HDAC6 antibody (CAB11259) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s. |
| Immunofluorescence analysis of HeLa cells using HDAC6 antibody (CAB11259) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
| Immunoprecipitation analysis of 100ug extracts of HepG2 cells using 3ug HDAC6 antibody (CAB11259). Western blot was performed from the immunoprecipitate using HDAC6 antibody (CAB11259) at a dilition of 1:1000. |
Datasheets
Additional Information
Antibody Type: |
Polyclonal |
Application: |
WB |
Application: |
IHC |
Application: |
IF |
Application: |
IP |
Reactivity: |
Human |
Reactivity: |
Mouse |
Reactivity: |
Rat |
Host Species: |
Rabbit |
Isotype: |
IgG |
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