Description
Title: | HDAC6 Polyclonal Antibody (CAB11259) | |
Size: | 100µL | |
Code: | CAB11259 | |
Host Species: | Rabbit | |
Purification: | Affinity purification | |
Isotype: | IgG |
Background
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription.
Immunogen Information
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 836-1104 of human HDAC6 (NP_006035.2). |
GeneID: | 10013 |
SwissProt: | Q9UBN7 |
Synonyms: | HDAC6; CPBHM; HD6; JM21; PPP1R90; histone deacetylase 6 |
Calculated MW: | 114kDa/131kDa |
Observed MW: | 170kDa |
Applications
Reactivity: | Human, Mouse, Rat |
Tested Applications: | WB; IHC; IF; IP |
Recommended Dilution: | WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200; IP 1:20 - 1:50 |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Application Key: | Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation |
Positive Samples: | U-87MG, SKOV3, HepG2, SW620, BT-474, Mouse kidney |
Cellular Location: | Cell projection, Cytoplasm, Nucleus, Perikaryon, axon, dendrite |
Antibody Images
Western blot analysis of extracts of various cell lines, using HDAC6 antibody (CAB11259) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s. | |
Immunofluorescence analysis of HeLa cells using HDAC6 antibody (CAB11259) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. | |
Immunoprecipitation analysis of 100ug extracts of HepG2 cells using 3ug HDAC6 antibody (CAB11259). Western blot was performed from the immunoprecipitate using HDAC6 antibody (CAB11259) at a dilition of 1:1000. |
Additional Information
Antibody Type: |
Polyclonal |
Application: |
WB |
Application: |
IHC |
Application: |
IF |
Application: |
IP |
Reactivity: |
Human |
Reactivity: |
Mouse |
Reactivity: |
Rat |
Host Species: |
Rabbit |
Isotype: |
IgG |