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HAAO Polyclonal Antibody (CAB4559)

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SKU:
CAB4559
€299.00

Description

Overview

Title:HAAO Polyclonal Antibody (CAB4559)
Size:100µL
Code:CAB4559
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

3-Hydroxyanthranilate 3, 4-dioxygenase is a monomeric cytosolic protein belonging to the family of intramolecular dioxygenases containing nonheme ferrous iron. It is widely distributed in peripheral organs, such as liver and kidney, and is also present in low amounts in the central nervous system. HAAO catalyzes the synthesis of quinolinic acid (QUIN) from 3-hydroxyanthranilic acid. QUIN is an excitotoxin whose toxicity is mediated by its ability to activate glutamate N-methyl-D-aspartate receptors. Increased cerebral levels of QUIN may participate in the pathogenesis of neurologic and inflammatory disorders. HAAO has been suggested to play a role in disorders associated with altered tissue levels of QUIN.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-286 of human HAAO (NP_036337.2).
GeneID:23498
SwissProt:P46952
Synonyms:HAAO; 3-HAO; HAO; 3-hydroxyanthranilate 3, 4-dioxygenase
Calculated MW:17kDa/32kDa
Observed MW:38kDa

Applications

Reactivity:Mouse, Rat
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:Mouse spleen, Rat liver
Cellular Location:Cytoplasm

Antibody Images

Western blot - HAAO Polyclonal Antibody (CAB4559)

Western blot analysis of extracts of various cell lines, using HAAO antibody (CAB4559) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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