GAPDH Monoclonal Antibody (CABC033)

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CABC033
  • Western blot - GAPDH Monoclonal Antibody (CABC033)
  • Immunohistochemistry - GAPDH Monoclonal Antibody (CABC033)
  • Immunofluorescence - GAPDH Monoclonal Antibody (CABC033)
  • Immunohistochemistry - GAPDH Monoclonal Antibody (CABC033)
  • Immunohistochemistry - GAPDH Monoclonal Antibody (CABC033)
  • Immunohistochemistry - GAPDH Monoclonal Antibody (CABC033)
  • Immunohistochemistry - GAPDH Monoclonal Antibody (CABC033)
  • Immunohistochemistry - GAPDH Monoclonal Antibody (CABC033)
  • Western blot - GAPDH Monoclonal Antibody (CABC033)
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Description

GAPDH Monoclonal Antibody (CABC033)

Overview

Title:GAPDH Monoclonal Antibody (CABC033)
Size:100uL
Code:CABC033
Host Species:Mouse
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.

Immunogen Information

Immunogen:Recombinant protein of human GAPDH
GeneID:2597
SwissProt:P04406
Synonyms:GAPDH; G3PD; GAPD; HEL-S-162eP; glyceraldehyde-3-phosphate dehydrogenase
Calculated MW:31kDa/36kDa
Observed MW:36kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF;
Recommended Dilution:WB 1:50000 - 1:200000; IHC 1:50 - 1:200; IF 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:HeLa, Jurkat, 293T, NIH/3T3, Raw 264.7, Mouse brain, Rat brain, Rat heart, Rat liver
Cellular Location:Cytoplasm, Membrane, Nucleus, cytoskeleton, cytosol, perinuclear region

Antibody Images

Western blot - GAPDH Monoclonal Antibody (CABC033)

Western blot analysis of extracts of various cell lines, using GAPDH antibody (CABC033) at 1:200000 dilution.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time:60s.

Western blot - GAPDH Monoclonal Antibody (CABC033)

Western blot analysis of extracts of HeLa cells, using GAPDH antibody (CABC033) at 1:10000-1:2560000 dilution.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.

Immunohistochemistry - GAPDH Monoclonal Antibody (CABC033)

Immunohistochemistry of paraffin-embedded rat spleen using GAPDH antibody (CABC033) at dilution of 1:100 (40x lens).

Immunohistochemistry - GAPDH Monoclonal Antibody (CABC033)

Immunohistochemistry of paraffin-embedded rat heart using GAPDH antibody (CABC033) at dilution of 1:100 (40x lens).

Immunohistochemistry - GAPDH Monoclonal Antibody (CABC033)

Immunohistochemistry of paraffin-embedded human tonsil using GAPDH antibody (CABC033) at dilution of 1:100 (40x lens).

Immunohistochemistry - GAPDH Monoclonal Antibody (CABC033)

Immunohistochemistry of paraffin-embedded human lung cancer using GAPDH antibody (CABC033) at dilution of 1:100 (40x lens).

Immunohistochemistry - GAPDH Monoclonal Antibody (CABC033)

Immunohistochemistry of paraffin-embedded human colon using GAPDH antibody (CABC033) at dilution of 1:100 (40x lens).

Immunohistochemistry - GAPDH Monoclonal Antibody (CABC033)

Immunohistochemistry of paraffin-embedded mouse spinal cord using GAPDH antibody (CABC033) at dilution of 1:100 (40x lens).

Immunofluorescence - GAPDH Monoclonal Antibody (CABC033)

Immunofluorescence analysis of PC-12 cells using GAPDH antibody (CABC033) at dilution of 1:200. Blue: DAPI for nuclear staining.

GAPDH Monoclonal Antibody (CABC033) Protein Information

UniProt Protein Function:GAPDH: a multifunctional enzyme with both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities. A key glycolytic enzyme that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. An important enzyme for energy metabolism, and the production of ATP and pyruvate through anaerobic glycolysis in the cytoplasm. Additionally, it participates in apoptosis, membrane trafficking, iron metabolism, nuclear activities and receptor mediated cell signaling. Its subcellular localization changes reflecting its multiple activities. Is cytosolic, but is also localized in the membrane, the nucleus, polysomes, the ER and the Golgi. Participates in transcription, RNA transport, DNA replication and apoptosis. S-nitrosylation on Cys-152 following apoptotic stimulates its interaction with SIAH2, which in turn moderates its translocation into the nucleus. Mediates cysteine S-nitrosylation of nuclear target proteins including SIRT1, HDAC2 and DNA-PK. Deregulated in lung cancer, renal cancer, breast cancer, gastric cancer, glioma, liver cancer, colorectal cancer, melanoma, prostatic cancer, pancreatic cancer and bladder cancer. Its increased expression and enzymatic activity is associated with cell proliferation and tumorigenesis, Oxidative stress impairs GAPDH catalytic activity and leads to cellular aging and apoptosis. In experimental animal models, injection of GAPDH antagonists induces apoptosis and blocks Hep3B tumor progression, suggesting a therapeutic potential of targeting GAPDH in human hepatocellular carcinoma
UniProt Protein Details:

Protein type:Oxidoreductase; EC 1.2.1.12; Carbohydrate Metabolism - glycolysis and gluconeogenesis

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: microtubule cytoskeleton; nuclear membrane; membrane; intracellular membrane-bound organelle; perinuclear region of cytoplasm; cytoplasm; plasma membrane; lipid particle; cytosol; ribonucleoprotein complex; nucleus; vesicle

Molecular Function:identical protein binding; protein binding; microtubule binding; glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity; NADP binding; NAD binding

Biological Process: neuron apoptosis; glycolysis; protein stabilization; negative regulation of translation; carbohydrate metabolic process; glucose metabolic process; pathogenesis; microtubule cytoskeleton organization and biogenesis; gluconeogenesis

NCBI Summary:This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]
UniProt Code:P04406
NCBI GenInfo Identifier:120649
NCBI Gene ID:2597
NCBI Accession:P04406.3
UniProt Secondary Accession:P04406,P00354, Q53X65, E7EUT4,
UniProt Related Accession:P04406
Molecular Weight:Calculated MW: 31kDa/36kDaObserved MW: 36kDa
NCBI Full Name:Glyceraldehyde-3-phosphate dehydrogenase
NCBI Synonym Full Names:glyceraldehyde-3-phosphate dehydrogenase
NCBI Official Symbol:GAPDH  
NCBI Official Synonym Symbols:G3PD; GAPD; HEL-S-162eP  
NCBI Protein Information:glyceraldehyde-3-phosphate dehydrogenase; aging-associated gene 9 protein; peptidyl-cysteine S-nitrosylase GAPDH; epididymis secretory sperm binding protein Li 162eP
UniProt Protein Name:Glyceraldehyde-3-phosphate dehydrogenase
UniProt Synonym Protein Names:Peptidyl-cysteine S-nitrosylase GAPDH (EC:2.6.99.-)
UniProt Gene Name:GAPDH  
UniProt Entry Name:G3P_HUMAN
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Additional Information

ANTIBODY TYPE:
Monoclonal
APPLICATION:
WB
APPLICATION:
IHC
APPLICATION:
IF
REACTIVITY:
Human
REACTIVITY:
Mouse
REACTIVITY:
Rat
HOST SPECIES:
Mouse
ISOTYPE:
IgG
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