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GAPDH Monoclonal Antibody (CABC033)

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SKU:
CABC033
  • Western blot - GAPDH Monoclonal Antibody (CABC033)
  • Immunohistochemistry - GAPDH Monoclonal Antibody (CABC033)
  • Immunofluorescence - GAPDH Monoclonal Antibody (CABC033)
  • Immunohistochemistry - GAPDH Monoclonal Antibody (CABC033)
  • Immunohistochemistry - GAPDH Monoclonal Antibody (CABC033)
  • Immunohistochemistry - GAPDH Monoclonal Antibody (CABC033)
  • Immunohistochemistry - GAPDH Monoclonal Antibody (CABC033)
  • Immunohistochemistry - GAPDH Monoclonal Antibody (CABC033)
  • Western blot - GAPDH Monoclonal Antibody (CABC033)
$331.89

Description

Overview

Title:GAPDH Monoclonal Antibody (CABC033)
Size:100uL
Code:CABC033
Host Species:Mouse
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.

Immunogen Information

Immunogen:Recombinant protein of human GAPDH
GeneID:2597
SwissProt:P04406
Synonyms:GAPDH; G3PD; GAPD; HEL-S-162eP; glyceraldehyde-3-phosphate dehydrogenase
Calculated MW:31kDa/36kDa
Observed MW:36kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF;
Recommended Dilution:WB 1:50000 - 1:200000; IHC 1:50 - 1:200; IF 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:HeLa, Jurkat, 293T, NIH/3T3, Raw 264.7, Mouse brain, Rat brain, Rat heart, Rat liver
Cellular Location:Cytoplasm, Membrane, Nucleus, cytoskeleton, cytosol, perinuclear region

Antibody Images

Western blot - GAPDH Monoclonal Antibody (CABC033)

Western blot analysis of extracts of various cell lines, using GAPDH antibody (CABC033) at 1:200000 dilution.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time:60s.

Western blot - GAPDH Monoclonal Antibody (CABC033)

Western blot analysis of extracts of HeLa cells, using GAPDH antibody (CABC033) at 1:10000-1:2560000 dilution.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.

Immunohistochemistry - GAPDH Monoclonal Antibody (CABC033)

Immunohistochemistry of paraffin-embedded rat spleen using GAPDH antibody (CABC033) at dilution of 1:100 (40x lens).

Immunohistochemistry - GAPDH Monoclonal Antibody (CABC033)

Immunohistochemistry of paraffin-embedded rat heart using GAPDH antibody (CABC033) at dilution of 1:100 (40x lens).

Immunohistochemistry - GAPDH Monoclonal Antibody (CABC033)

Immunohistochemistry of paraffin-embedded human tonsil using GAPDH antibody (CABC033) at dilution of 1:100 (40x lens).

Immunohistochemistry - GAPDH Monoclonal Antibody (CABC033)

Immunohistochemistry of paraffin-embedded human lung cancer using GAPDH antibody (CABC033) at dilution of 1:100 (40x lens).

Immunohistochemistry - GAPDH Monoclonal Antibody (CABC033)

Immunohistochemistry of paraffin-embedded human colon using GAPDH antibody (CABC033) at dilution of 1:100 (40x lens).

Immunohistochemistry - GAPDH Monoclonal Antibody (CABC033)

Immunohistochemistry of paraffin-embedded mouse spinal cord using GAPDH antibody (CABC033) at dilution of 1:100 (40x lens).

Immunofluorescence - GAPDH Monoclonal Antibody (CABC033)

Immunofluorescence analysis of PC-12 cells using GAPDH antibody (CABC033) at dilution of 1:200. Blue: DAPI for nuclear staining.
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Additional Information

ANTIBODY TYPE:
Monoclonal
APPLICATION:
WB
APPLICATION:
IHC
APPLICATION:
IF
REACTIVITY:
Human
REACTIVITY:
Mouse
REACTIVITY:
Rat
HOST SPECIES:
Mouse
ISOTYPE:
IgG
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