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GAPDH Monoclonal Antibody (CABC002)

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SKU:
CABC002
$298.59

Description

Overview

Title:GAPDH Monoclonal Antibody (CABC002)
Size:100uL
Code:CABC002
Host Species:Mouse
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.

Immunogen Information

Immunogen:Recombinant protein of human GAPDH
GeneID:2597
SwissProt:P04406
Synonyms:GAPDH; G3PD; GAPD; HEL-S-162eP; glyceraldehyde-3-phosphate dehydrogenase
Calculated MW:31kDa/36kDa
Observed MW:36kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF;
Recommended Dilution:WB 1:5000 - 1:20000; IHC 1:50 - 1:200; IF 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:HeLa, HepG2, MCF7, Mouse liver, Mouse heart, Mouse brain, Rat heart, Rat brain
Cellular Location:Cytoplasm, Membrane, Nucleus, cytoskeleton, cytosol, perinuclear region

Antibody Images

Western blot - GAPDH Monoclonal Antibody (CABC002)

Western blot analysis of extracts of various cell lines, using GAPDH antibody (CABC002) at 1:10000 dilution.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
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Additional Information

ANTIBODY TYPE:
Monoclonal
APPLICATION:
WB
APPLICATION:
IHC
APPLICATION:
IF
REACTIVITY:
Human
REACTIVITY:
Mouse
REACTIVITY:
Rat
HOST SPECIES:
Mouse
ISOTYPE:
IgG
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