FLT1 Polyclonal Antibody (CAB0058)

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CAB0058
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Description

Antibody DatasheetAntibody MSDS

FLT1 Polyclonal Antibody (CAB0058)

Overview

Title:FLT1 Polyclonal Antibody (CAB0058)
Size:100µL
Code:CAB0058
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a member of the vascular endothelial growth factor receptor (VEGFR) family. VEGFR family members are receptor tyrosine kinases (RTKs) which contain an extracellular ligand-binding region with seven immunoglobulin (Ig)-like domains, a transmembrane segment, and a tyrosine kinase (TK) domain within the cytoplasmic domain. This protein binds to VEGFR-A, VEGFR-B and placental growth factor and plays an important role in angiogenesis and vasculogenesis. Expression of this receptor is found in vascular endothelial cells, placental trophoblast cells and peripheral blood monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Isoforms include a full-length transmembrane receptor isoform and shortened, soluble isoforms. The soluble isoforms are associated with the onset of pre-eclampsia.

Immunogen Information

Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1100-1200 of human FLT1 (NP_002010.2).
GeneID:2321
SwissProt:P17948
Synonyms:FLT1; FLT; FLT-1; VEGFR-1; VEGFR1; fms related tyrosine kinase 1
Calculated MW:39kDa/41kDa/52- 82kDa/150kDa
Observed MW:43kDa

Applications

Reactivity:Human
Tested Applications:WB
Recommended Dilution:WB 1:200 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:LO2
Cellular Location:Cell membrane, Cytoplasm, Endosome, Secreted, Single-pass type I membrane protein

Antibody Images

Western blot - FLT1 Polyclonal Antibody (CAB0058)

Western blot analysis of extracts of LO2 cells, using FLT1 antibody (CAB0058) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

FLT1 Polyclonal Antibody (CAB0058) Protein Information

UniProt Protein Function:VEGFR1: a receptor tyrosine kinase of the VEGFR family. Receptor for VEGF, VEGFB and PGF. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Two splice variant isoforms have been described. Isoform SFlt1 may have an inhibitory role in angiogenesis.
UniProt Protein Details:

Protein type:Protein kinase, TK; EC 2.7.10.1; Protein kinase, tyrosine (receptor); Kinase, protein; Membrane protein, integral; TK group; VEGFR family

Chromosomal Location of Human Ortholog: 13q12

Cellular Component: extracellular space; focal adhesion; integral to plasma membrane; plasma membrane; receptor complex; endosome

Molecular Function:identical protein binding; vascular endothelial growth factor receptor activity; protein binding; growth factor binding; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: cell migration; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase activity; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of phosphoinositide 3-kinase cascade; patterning of blood vessels; monocyte chemotaxis; positive regulation of MAP kinase activity; positive regulation of angiogenesis; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; blood vessel morphogenesis; embryonic morphogenesis; sprouting angiogenesis; cell differentiation; vascular endothelial growth factor receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration

NCBI Summary:This gene encodes a member of the vascular endothelial growth factor receptor (VEGFR) family. VEGFR family members are receptor tyrosine kinases (RTKs) which contain an extracellular ligand-binding region with seven immunoglobulin (Ig)-like domains, a transmembrane segment, and a tyrosine kinase (TK) domain within the cytoplasmic domain. This protein binds to VEGFR-A, VEGFR-B and placental growth factor and plays an important role in angiogenesis and vasculogenesis. Expression of this receptor is found in vascular endothelial cells, placental trophoblast cells and peripheral blood monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Isoforms include a full-length transmembrane receptor isoform and shortened, soluble isoforms. The soluble isoforms are associated with the onset of pre-eclampsia.[provided by RefSeq, May 2009]
UniProt Code:P17948
NCBI GenInfo Identifier:143811474
NCBI Gene ID:2321
NCBI Accession:P17948.2
UniProt Secondary Accession:P17948,O60722, A3E342, A3E344, A8KA71, B0LPF1, B2BF46 B2BF47, B2BF48, B3FR89, B5A923, F5H5L6,
UniProt Related Accession:P17948
Molecular Weight:41,175 Da
NCBI Full Name:Vascular endothelial growth factor receptor 1
NCBI Synonym Full Names:fms-related tyrosine kinase 1
NCBI Official Symbol:FLT1  
NCBI Official Synonym Symbols:FLT; FLT-1; VEGFR1; VEGFR-1  
NCBI Protein Information:vascular endothelial growth factor receptor 1; fms-like tyrosine kinase 1; tyrosine-protein kinase FRT; tyrosine-protein kinase receptor FLT; vascular permeability factor receptor; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)
UniProt Protein Name:Vascular endothelial growth factor receptor 1
UniProt Synonym Protein Names:Fms-like tyrosine kinase 1; FLT-1; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; FLT; Vascular permeability factor receptor
Protein Family:Vascular endothelial growth factor receptor
UniProt Gene Name:FLT1  
UniProt Entry Name:VGFR1_HUMAN
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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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