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FLJ20294 Polyclonal Antibody (CAB1083)

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SKU:
CAB1083
  • Western blot - FLJ20294 Polyclonal Antibody (CAB1083)
  • Immunohistochemistry - FLJ20294 Polyclonal Antibody (CAB1083)
  • Immunohistochemistry - FLJ20294 Polyclonal Antibody (CAB1083)
  • Immunohistochemistry - FLJ20294 Polyclonal Antibody (CAB1083)
  • Immunohistochemistry - FLJ20294 Polyclonal Antibody (CAB1083)
  • Immunofluorescence - FLJ20294 Polyclonal Antibody (CAB1083)
$331.89

Description

Overview

Title:FLJ20294 Polyclonal Antibody (CAB1083)
Size:100µL
Code:CAB1083
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

N/A

Immunogen Information

Immunogen:Recombinant protein of human AMBRA1
GeneID:55626
SwissProt:Q9C0C7
Synonyms:AMBRA1; DCAF3; WDR94; autophagy and beclin 1 regulator 1
Calculated MW:84kDa/132kDa/135kDa/139kDa/142kDa
Observed MW:143kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:U-87MG, HeLa, Jurkat, MCF7
Cellular Location:Cytoplasmic vesicle, autophagosome

Antibody Images

Western blot - FLJ20294 Polyclonal Antibody (CAB1083)

Western blot analysis of extracts of various cell lines, using FLJ20294 Antibody (CAB1083) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.

Immunohistochemistry - FLJ20294 Polyclonal Antibody (CAB1083)

Immunohistochemistry of paraffin-embedded mouse stomach using AMBRA1 antibody (CAB1083) at dilution of 1:100 (40x lens).

Immunohistochemistry - FLJ20294 Polyclonal Antibody (CAB1083)

Immunohistochemistry of paraffin-embedded rat lung using AMBRA1 antibody (CAB1083) at dilution of 1:100 (40x lens).

Immunohistochemistry - FLJ20294 Polyclonal Antibody (CAB1083)

Immunohistochemistry of paraffin-embedded rat kidney using AMBRA1 antibody (CAB1083) at dilution of 1:100 (40x lens).

Immunohistochemistry - FLJ20294 Polyclonal Antibody (CAB1083)

Immunohistochemistry of paraffin-embedded mouse lung using AMBRA1 antibody (CAB1083) at dilution of 1:100 (40x lens).

Immunofluorescence - FLJ20294 Polyclonal Antibody (CAB1083)

Immunofluorescence analysis of HeLa cells using FLJ20294 antibody (CAB1083) at dilution of 1:100. Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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