FAM65B Polyclonal Antibody (CAB9234)

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CAB9234
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Description

Antibody DatasheetAntibody MSDS

FAM65B Polyclonal Antibody (CAB9234)

Overview

Title:FAM65B Polyclonal Antibody (CAB9234)
Size:100µL
Code:CAB9234
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes an atypical inhibitor of the small G protein RhoA. Inhibition of RhoA activity by the encoded protein mediates myoblast fusion and polarization of T cells and neutrophils. The encoded protein is a component of hair cell stereocilia that is essential for hearing. A splice site mutation in this gene results in hearing loss in human patients.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human FAM65B (NP_056948.2).
GeneID:9750
SwissProt:Q9Y4F9
Synonyms:RIPOR2; C6orf32; DFNB104; DIFF40; DIFF48; FAM65B; MYONAP; PL48; protein FAM65B
Calculated MW:65kDa/118kDa
Observed MW:118kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:U-87MG, Mouse thymus, Mouse brain, Rat thymus, Rat brain
Cellular Location:Cell projection, Cytoplasm, Mitochondrion, cytoskeleton, filopodium, stereocilium membrane

Antibody Images

Western blot - FAM65B Polyclonal Antibody (CAB9234)

Western blot analysis of extracts of various cell lines, using FAM65B antibody (CAB9234) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

FAM65B Polyclonal Antibody (CAB9234) Protein Information

UniProt Protein Function:FAM65B: Isoform 2 play a role in promoting myogenic cell differentiation, cytoskeletal rearrangement and filopodia formation. Belongs to the FAM65 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cytoskeletal

Chromosomal Location of Human Ortholog: 6p22.3-p21.32

Cellular Component: cytoplasm

Molecular Function:protein binding

Biological Process: sensory perception of sound

Disease: Deafness, Autosomal Recessive 104

NCBI Summary:The protein encoded by this gene stimulates the formation of a non-mitotic multinucleate syncytium from proliferative cytotrophoblasts during trophoblast differentiation. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2013]
UniProt Code:Q9Y4F9
NCBI GenInfo Identifier:296439477
NCBI Gene ID:9750
NCBI Accession:Q9Y4F9.4
UniProt Secondary Accession:Q9Y4F9,Q13529, Q5VV37, Q5VV38, Q9BQ28, A6NHP2,
UniProt Related Accession:Q9Y4F9
Molecular Weight:65,692 Da
NCBI Full Name:Protein FAM65B
NCBI Synonym Full Names:family with sequence similarity 65 member B
NCBI Official Symbol:FAM65B  
NCBI Official Synonym Symbols:PL48; DIFF40; DIFF48; MYONAP; C6orf32; DFNB104  
NCBI Protein Information:protein FAM65B
UniProt Protein Name:Protein FAM65B
Protein Family:Protein
UniProt Gene Name:FAM65B  
UniProt Entry Name:FA65B_HUMAN
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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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