The cookie settings on this website are set to 'allow all cookies' to give you the very best experience. Please click Accept Cookies to continue to use the site.

ERVW-1 Polyclonal Antibody (CAB16522)

(No reviews yet) Write a Review
SKU:
CAB16522
$331.89

Description

Overview

Title:ERVW-1 Polyclonal Antibody (CAB16522)
Size:100µL
Code:CAB16522
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

Many different human endogenous retrovirus (HERV) families are expressed in normal placental tissue at high levels, suggesting that HERVs are functionally important in reproduction. This gene is part of an HERV provirus on chromosome 7 that has inactivating mutations in the gag and pol genes. This gene is the envelope glycoprotein gene which appears to have been selectively preserved. The gene's protein product is expressed in the placental syncytiotrophoblast and is involved in fusion of the cytotrophoblast cells to form the syncytial layer of the placenta. The protein has the characteristics of a typical retroviral envelope protein, including a furin cleavage site that separates the surface (SU) and transmembrane (TM) proteins which form a heterodimer. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

Immunogen Information

Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ERVW-1 (NP_001124397.1).
GeneID:30816
SwissProt:Q9UQF0
Synonyms:ERVW-1; ENV; ENVW; ERVWE1; HERV-7q; HERV-W-ENV; HERV7Q; HERVW; HERVWENV; syncytin-1
Calculated MW:59kDa
Observed MW:55kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:U-251MG, 293T, mouse brain
Cellular Location:Cell membrane, Peripheral membrane protein, Single-pass type I membrane protein, Virion

Antibody Images

Western blot - ERVW-1 Polyclonal Antibody (CAB16522)

Western blot analysis of extracts of various cell lines, using ERVW-1 antibody (CAB16522) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.

Datasheets

Antibody DatasheetAntibody MSDS

View AllClose

Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
View AllClose