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ERCC1 Monoclonal Antibody (CAB0777)

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SKU:
CAB0777
$331.89

Description

Overview

Title:ERCC1 Monoclonal Antibody (CAB0777)
Size:100µL
Code:CAB0777
Host Species:Mouse
Purification:Affinity purification
Isotype:IgG

Background

The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand.

Immunogen Information

Immunogen:A synthetic peptide of human ERCC1
GeneID:2067
SwissProt:P07992
Synonyms:ERCC1; COFS4; RAD10; UV20; DNA excision repair protein ERCC-1
Calculated MW:25kDa/29kDa/32kDa/35kDa
Observed MW:39kDa

Applications

Reactivity:Human
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at 4°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, pH7.3.
Application Key:Western blotting    
Positive Samples:MCF7
Cellular Location:Cytoplasm, Nucleus

Antibody Images

Western blot - ERCC1 Monoclonal Antibody (CAB0777)

Western blot analysis of extracts of MCF-7 cells, using ERCC1 antibody (CAB0777).Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Monoclonal
Application:
WB
Reactivity:
Human
Host Species:
Mouse
Isotype:
IgG
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