DKK2 Polyclonal Antibody (CAB14874)

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CAB14874
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Description

Antibody DatasheetAntibody MSDS

DKK2 Polyclonal Antibody (CAB14874)

Overview

Title:DKK2 Polyclonal Antibody (CAB14874)
Size:100µL
Code:CAB14874
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. It can act as either an agonist or antagonist of Wnt/beta-catenin signaling, depending on the cellular context and the presence of the co-factor kremen 2. Activity of this protein is also modulated by binding to the Wnt co-receptor LDL-receptor related protein 6 (LRP6).

Immunogen Information

Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human DKK2 (NP_055236.1).
GeneID:27123
SwissProt:Q9UBU2
Synonyms:DKK2; DKK-2; dickkopf-related protein 2
Calculated MW:28kDa
Observed MW:30kDa

Applications

Reactivity:Human
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:A549, H460
Cellular Location:Secreted

Antibody Images

Western blot - DKK2 Polyclonal Antibody (CAB14874)

Western blot analysis of extracts of various cell lines, using DKK2 antibody (CAB14874) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

DKK2 Polyclonal Antibody (CAB14874) Protein Information

UniProt Protein Function:DKK2: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Interacts with LRP5 and LRP6. Expressed in heart, brain, skeletal muscle and lung. Belongs to the dickkopf family.
UniProt Protein Details:

Protein type:Secreted; Secreted, signal peptide; Inhibitor

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: extracellular space

NCBI Summary:This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. It can act as either an agonist or antagonist of Wnt/beta-catenin signaling, depending on the cellular context and the presence of the co-factor kremen 2. Activity of this protein is also modulated by binding to the Wnt co-receptor LDL-receptor related protein 6 (LRP6). [provided by RefSeq, Jul 2008]
UniProt Code:Q9UBU2
NCBI GenInfo Identifier:13124093
NCBI Gene ID:27123
NCBI Accession:Q9UBU2.1
UniProt Secondary Accession:Q9UBU2,Q9UIU3, A0AVE9, B2R6S7,
UniProt Related Accession:Q9UBU2
Molecular Weight:28,447 Da
NCBI Full Name:Dickkopf-related protein 2
NCBI Synonym Full Names:dickkopf WNT signaling pathway inhibitor 2
NCBI Official Symbol:DKK2  
NCBI Official Synonym Symbols:DKK-2  
NCBI Protein Information:dickkopf-related protein 2
UniProt Protein Name:Dickkopf-related protein 2
Protein Family:Dickkopf-related protein
UniProt Gene Name:DKK2  
UniProt Entry Name:DKK2_HUMAN
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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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