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DHFR Polyclonal Antibody (CAB1607)

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CAB1607
  • Western blot - DHFR Polyclonal Antibody (CAB1607)
  • Immunofluorescence - DHFR Polyclonal Antibody (CAB1607)
$331.89

Description

Overview

Title:DHFR Polyclonal Antibody (CAB1607)
Size:100µL
Code:CAB1607
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-187 of human DHFR (NP_000782.1).
GeneID:1719
SwissProt:P00374
Synonyms:DHFR; DHFRP1; DYR; dihydrofolate reductase
Calculated MW:15kDa/21kDa
Observed MW:19kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB; IF
Recommended Dilution:WB 1:500 - 1:2000; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    
Positive Samples:HeLa, 22Rv1, K-562, HL-60, THP-1, Raji, Mouse liver
Cellular Location:N/A

Antibody Images

Western blot - DHFR Polyclonal Antibody (CAB1607)

Western blot analysis of extracts of various cell lines, using DHFR antibody (CAB1607) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence - DHFR Polyclonal Antibody (CAB1607)

Immunofluorescence analysis of A-549 cells using DHFR antibody (CAB1607).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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