DHFR Polyclonal Antibody (CAB1607)

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CAB1607
  • Western blot - DHFR Polyclonal Antibody (CAB1607)
  • Immunofluorescence - DHFR Polyclonal Antibody (CAB1607)
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Description

Antibody DatasheetAntibody MSDS

DHFR Polyclonal Antibody (CAB1607)

Overview

Title:DHFR Polyclonal Antibody (CAB1607)
Size:100µL
Code:CAB1607
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-187 of human DHFR (NP_000782.1).
GeneID:1719
SwissProt:P00374
Synonyms:DHFR; DHFRP1; DYR; dihydrofolate reductase
Calculated MW:15kDa/21kDa
Observed MW:19kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB; IF
Recommended Dilution:WB 1:500 - 1:2000; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    
Positive Samples:HeLa, 22Rv1, K-562, HL-60, THP-1, Raji, Mouse liver
Cellular Location:N/A

Antibody Images

Western blot - DHFR Polyclonal Antibody (CAB1607)

Western blot analysis of extracts of various cell lines, using DHFR antibody (CAB1607) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence - DHFR Polyclonal Antibody (CAB1607)

Immunofluorescence analysis of A-549 cells using DHFR antibody (CAB1607).

DHFR Polyclonal Antibody (CAB1607) Protein Information

UniProt Protein Function:DHFR: Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1. Defects in DHFR are the cause of megaloblastic anemia due to dihydrofolate reductase deficiency (DHFRD). DHFRD is an inborn error of metabolism, characterized by megaloblastic anemia and/or pancytopenia, severe cerebral folate deficiency, and cerebral tetrahydrobiopterin deficiency. Clinical features include variable neurologic symptoms, ranging from severe developmental delay and generalized seizures in infancy, to childhood absence epilepsy with learning difficulties, to lack of symptoms. Belongs to the dihydrofolate reductase family.
UniProt Protein Details:

Protein type:Cofactor and Vitamin Metabolism - one carbon pool by folate; Oxidoreductase; EC 1.5.1.3; Cofactor and Vitamin Metabolism - folate biosynthesis

Chromosomal Location of Human Ortholog: 5q14.1

Cellular Component: nucleoplasm; cytosol

Molecular Function:mRNA binding; drug binding; dihydrofolate reductase activity; NADP binding

Biological Process: tetrahydrofolate metabolic process; vitamin metabolic process; one-carbon compound metabolic process; nitric oxide metabolic process; G1/S-specific transcription in mitotic cell cycle; tetrahydrofolate biosynthetic process; nucleotide biosynthetic process; response to methotrexate; regulation of nitric-oxide synthase activity; folic acid metabolic process; mitotic cell cycle; glycine biosynthetic process; water-soluble vitamin metabolic process; G1/S transition of mitotic cell cycle

Disease: Megaloblastic Anemia Due To Dihydrofolate Reductase Deficiency

UniProt Code:P00374
NCBI GenInfo Identifier:118992
NCBI Gene ID:
NCBI Accession:P00374.2
Molecular Weight:
NCBI Full Name:Dihydrofolate reductase
UniProt Protein Name:Dihydrofolate reductase
Protein Family:Viral dihydrofolate reductase
UniProt Gene Name:DHFR  
UniProt Entry Name:DYR_HUMAN
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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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